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Neutrophil Elastase Inhibitor Screening Kit (Fluorometric)

Catalog #: K782
$465.00

Product Details

Cat # +Size K782-100
Size 100 assays
Detection Method Fluorescence (Ex/Em 400/505 nm)
Applications This kit can be used for screening NE inhibitors and comes along with positive and negative control inhibitors
Features & Benefits • Simple procedure; takes ~ less than 40 minutes
• Fast and convenient
Kit Components • Assay Buffer
• Substrate
• Neutrophil Elastase
• Inhibitor Control (3 mM, SPCK)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Neutrophil Elastase (NE) is an aggressive and cytotoxic 29 kDa serine protease stored mainly in the azurophil granules of neutrophil granulocytes. It plays a role in the degradation of a wide range of extracellular matrix proteins, including fibronectin, laminin, proteoglycans, collagens, and elastin. When the extracellular NE concentration exceeds the buffering capacity of endogenous inhibitors, it becomes implicated in the signs, symptoms and disease progression of inflammatory lung disorders via its role in the inflammatory process, mucus overproduction and lung tissue damage. In BioVision’s Neutrophil Elastase Inhibitor Screening Kit, NE hydrolyzes a specific fluorescent substrate to release the fluorescent group, which can be detected at Ex/Em = 400/505 nm. In presence of a potent Neutrophil Elastase inhibitor, the hydrolyzation of substrate will be inhibited or stopped. The kit provides a rapid, simple, sensitive, and reliable test suitable as a high throughput screening assay of Neutrophil Elastase inhibitors. For comparison of the relative efficacy of test inhibitors, a control inhibitor, SPCK (Ki = 10 µM for human leukocyte elastase) is included.


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According to the datasheet, the test compounds should be dissolved with the proper solvent However, is there any recommended solvent for this assay?
You can use any solvent that will dissolve your compound of interest. To make sure that the solvent does not interfere with the reaction, please include a solvent control in the assay. Please also note that it is recommended not to exceed the solvent volume by more than 2% of your total reaction volume.