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Neprilysin (Human) ELISA Kit

A Sandwich ELISA kit for the quantitative measurement of Neprilysin in human serum, plasma, tissue lysates, and other biological fluids
Catalog #: E4882
$695.00

Product Details

Cat # +Size E4882-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications This Sandwich ELISA kit is designed to quantitatively measure amount of Neprilysin in human serum, plasma, tissue lysates and other biological fluids
Features & Benefits • Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%
• Recovery range: 85 - 104% for normal human serum and plasma samples
• Sensitivity: 0.094 ng/ml
• This Sandwich ELISA is highly sensitive and highly specific for the detection of Neprilysin in human samples. There is no significant cross-reactivity or interference between Neprilysin and analogues
• Detection range: 0.156 - 10 ng/ml
Kit Components • Micro ELISA plate
• Standard (Lyophilized) (10 ng)
• Sample/Standard Dilution Buffer
• Biotin-labeled Antibody
• Antibody Dilution Buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC Dilution Buffer
• TMB Substrate Solution
• Stop Solution
• Wash Buffer (25X)
• Plate Sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Neprilysin (MME, CD10) is a zinc-dependent membrane metallopeptidase located on the surface of neutrophils, endothelial cells, and a variety of tissues such as brain, heart, kidneys, and lungs. It catalyzes the degradation of peptides such as atrial (ANP), B-type (BNP), and C-type (CNP) natriuretic peptides, Angiotensin-II, Bradykinin, and Endothelin-1. It also degrades amyloid-beta peptide in the brain and slows the progression of Alzheimer’s disease. Additionally, it is an important cell surface marker in the diagnosis of hematologic diseases such as human acute lymphocytic leukemia (ALL), Burkitt lymphoma, and diffuse large B-cell lymphoma. BioVision’s Neprilysin (Human) ELISA kit is based on the Sandwich ELISA principle to quantitatively measure the amount of Neprilysin in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and subsequently washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed away with Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of Neprilysin in the sample or standard.


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