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Neopterin (Human) ELISA Kit

A Competitive ELISA Kit to quantitatively measure Neopterin in human serum, plasma, tissue lysates, and other biological fluids

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which is [are] known to the State of California to cause birth defects or other reproductive harm.  For more information go to  www.P65Warnings.ca.gov.
Catalog #: E4928
$695.00

Product Details

Cat # +Size E4928-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications This Competitive ELISA kit is designed to quantitatively measure amount of Neopterin in human serum, plasma, tissue lysates, and other biological fluids
Features & Benefits • Detection range: 0.156 - 10 ng/ml
• This Competitive ELISA is highly sensitive and highly specific for the detection of Neopterin in human samples. There is no significant cross-reactivity or interference between Neopterin and analogues
• Sensitivity: 0.094 ng/ml
• Recovery range: 85 - 105% for normal human serum and plasma samples
• Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%
Kit Components • Micro ELISA plate
• Standard (Lyophilized) (10 ng)
• Sample/Standard Dilution Buffer
• Biotin-labeled Antibody
• Antibody Dilution Buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC Dilution Buffer
• TMB Substrate Solution
• Stop Solution
• Wash Buffer (25X)
• Plate Sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Neopterin (NP) is a 253 Da pyrazinopyridine derivative that is produced from guanosine triphosphate by the action of the enzyme guanosine triphosphate cyclohydrolase I. Upon stimulation by interferon-γ, the pteridine derivative is produced by multiple cells such as activated monocytes, dendritic cells, and macrophages. It is also produced to a lesser extent by fibroblasts, vascular smooth muscle cells, and renal epithelial cells. Neopterin can be detected in a variety of samples such as serum, urine, CSF, saliva, and synovial fluid. Elevated levels are observed during allograft rejections, bacterial and viral infections, autoimmune diseases, and cancer. Thus, Neopterin could serve as a useful marker for the prognosis of human diseases. BioVision’s Neopterin (Human) ELISA kit is based on the Competitive ELISA principle to quantitatively measure the concentration of Neopterin in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is inversely proportional to the concentration of Neopterin in the sample or standard.


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