NADH Oxidase Activity Assay Kit (Fluorometric) (ab273345)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
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Product name
NADH Oxidase Activity Assay Kit (Fluorometric)
See all NADH Oxidase kits -
Detection method
Fluorescent -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay type
Enzyme activity (quantitative) -
Assay duration
Multiple steps standard assay -
Product overview
NADH Oxidase Activity Assay Kit (Fluorometric) (ab273345) couples oxidation of NADH by NADH Oxidase (NOX) and reduction of a colorless probe to a brightly colored product generating fluorescence at Ex/Em = 535/587 nm. The fluorescence generated is directly proportional to the NOX activity in samples.
Lactate Standard ranges from 0-250 pmol/well.
The kit can be used to determine NOX activity in a variety of sample type with a detection limit of ~ 15 µU of NOX activity per reaction.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K2019 NADH Oxidase Activity Assay Kit (Fluorometric). K2019-100 is the same size as the 100 test size of ab273345.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Assay Buffer V 1 x 25ml Assay Buffer XII 1 x 25ml L(+)-Lactate Standard 1 x 100µl NADH Oxidase Developer 2 vials Enzyme Mix XV 1 vial NADH Oxidase Positive Control 1 vial NADH Oxidase Substrate I 1 x 20µl NADH Oxidase Substrate II 1 x 300µl PicoProbe I 1 x 400µl
Images
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Example of Lactate Standard Curve.
One mole of Lactate corresponds to one mole of β-NAD+ reduced to NADH, which subsequently generates one mole of reduced substrate.
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Example data.
Reaction kinetics of recombinant NADH oxidase (Positive Control) and HepG2 cell lysate.
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Example data.
NADH oxidase activity detected in rat liver lysate (1.3μg total protein), HepG2 cell lysate (6μg total protein) and HeLa cell lysate (0.86μg total protein).
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab273345 has not yet been referenced specifically in any publications.