Call 408.493.1800 | Fax 408.493.1801 | Toll Free 800.891.9699 (US Only) | Email: [email protected]

NADH Oxidase Activity Assay Kit (Fluorometric)

to measure NADH oxidase activity
Catalog #: K2019
$495.00

Product Details

Cat # +Size K2019-100
Size 100 assays
Kit Summary BioVision’s NADH Oxidase Activity Assay Kit couples oxidation of NADH by NOX and reduction of a colorless probe to a brightly colored product generating fluorescence at Ex/Em = 535/587 nm. The fluorescence generated is directly proportional to the NOX activity in samples. The kit can be used to determine NOX activity in a variety of sample type with a detection limit of ~ 15 µU of NOX activity per reaction.
Detection Method Fluorescence (Ex/Em = 535/587 nm)
Species Reactivity Mammalian
Applications • Analysis of ROS in different samples
• Mechanistic studies of various cancers
• Measurement of NADH oxidase activity in various tissues/cells
• Screening anti-cancer drugs
Features & Benefits • Simple
• Rapid
• Can be used to determine NOX activity in a variety of sample type with a detection limit of ~ 15 µU of NOX activity per reaction.
Kit Components NADH Oxidase Lysis Buffer
NADH Oxidase Positive Control
L (+) Lactate Standard
NADH Oxidase Assay Buffer
NADH Oxidase Substrate II
NADH Oxidase Substrate I
NADH Oxidase Developer
NADH Oxidase Enzyme mix
PicoProbe™
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

NADH oxidase (Nicotinamide Adenine Dinucleotide Phosphate Oxidase, NOX) is a homotetrameric flavoenzyme that catalyzes the oxidation of NADH by molecular oxygen to yield NAD+. NOX is one of the well-known cancer biomarkers and is shown to be stimulated by growth factors and hormones. Several studies revealed the high activity of NADH oxidase in cancer patients as compared to healthy volunteers. More importantly, inhibition of NOX correlates with reduced cancer cell growth and induction of apoptosis. RNA interference targeting NOX expression in cancer cells effectively restored the non-cancerous phenotypes thereby further supporting the vital role of NOX in cancer progression. Furthermore, NADH oxidase is critical for the regeneration of NAD+, for use in glycolysis, and for the reduction of oxygen thereby preventing the formation of damaging reactive oxygen species. This makes NADH oxidase an attractive therapeutic target. BioVision’s NADH Oxidase Activity Assay Kit couples oxidation of NADH by NOX and reduction of a colorless probe to a brightly colored product generating fluorescence at Ex/Em = 535/587 nm. The fluorescence generated is directly proportional to the NOX activity in samples. The kit can be used to determine NOX activity in a variety of sample type with a detection limit of ~ 15 µU of NOX activity per reaction.


Why buy BioVision Products?
Innovation
Affordability
Global Presence
Technical Support
BioVision aims to provide our customers innovative tools for accelerating drug discovery and biological research. BioVision offers >8,000 products including the most comprehensive array of assay kits for key targets in Metabolic pathways.
BioVision is committed to providing the highest quality products at a competitive price.
We have a broad network of global distributors who are ready to address your research needs and ensure fast delivery.
Our highly trained Technical Support team provides comprehensive product support and is dedicated to resolving your issues quickly and efficiently.