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NADH Oxidase Activity Assay Kit (Colorimetric)

to determine the NADH Oxidase activity in a variety of sample types
Catalog #: K2028

Product Details

Size 100 assay
Detection Method Absorbance, OD 600 nm
Species Reactivity Mammalian
Applications • Mechanistic studies of various cancers
• Screening anti-cancer drugs
• Measurement of NADH oxidase activity in various tissues/cells
• Analysis of ROS in various samples
Features & Benefits • Simple
• Rapid
• Can be used to determine NOX activity in a variety of sample types with a detection limit of ~ 1 mU of NOX activity per reaction.
Kit Components • NADH Oxidase Assay Buffer
• NADH Oxidase Probe
• NADH Oxidase Enzyme mix
• NADH Oxidase Substrate I
• NADH Oxidase Substrate II
• NADH Oxidase Positive Control
• NADH Oxidase Standard
Storage Conditions -20ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


NADH oxidase (Nicotinamide Adenine Dinucleotide Phosphate Oxidase, NOX) is a flavoprotein that catalyzes the oxidation of NADH by molecular oxygen to yield NAD+. NOX is one of the well-known cancer biomarkers and is shown to be stimulated by growth factors and hormones. Several studies revealed the high activity of NADH oxidase in cancer patients as compared to the healthy volunteers. More importantly, inhibition of NOX correlates with the reduced cancer cell growth and induction of apoptosis. RNA interference targeting NOX expression in cancer cells effectively restored the non-cancerous phenotypes, further supporting the vital role of NOX in cancer progression. Furthermore, NOX is critical for the regeneration of NAD+, for use in glycolysis and for the reduction of oxygen thereby preventing the formation of damaging reactive oxygen species. This makes NADH oxidase an attractive therapeutic target. BioVision’s NADH Oxidase Activity Assay Kit couples the oxidation of NADH by NOX and the reduction of a colored substrate to a colorless product. This result in the decrease of absorbance measured at 600 nm. The kit can be used to determine NOX activity in a variety of sample types with a detection limit of ~ 1 mU of NOX activity per reaction.

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