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Product Details
| Cat # +Size | K822-100 |
|---|---|
| Size | 100 assays |
| Detection Method | Fluorescence (Ex/Em = 392/482 nm) |
| Species Reactivity | Mammalian |
| Applications | Screening/studying/characterizing potential inhibitors of NNMT |
| Features & Benefits | • Rapid assay and simple protocol • Reliable test for screening potential inhibitors of NNMT • Includes Inhibitor Control, 1-Methylnicotinamide (MNA) |
| Kit Components | • NNMT Assay Buffer • NNMT Enzyme • S-Adenosylmethionine (SAM) • Nicotinamide • Enzyme-I • Enzyme-II • 1-Methylnicotinamide (MNA) (150 mM) • Thiol Detecting Probe (DMSO) • SAM Reconstitution Buffer |
| Storage Conditions | -80°C |
| Shipping Conditions | Dry Ice |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
N′-Nicotinamide Methyltransferase (NNMT) (E.C. 2.1.1.1) catalyzes the N-methylation of nicotinamide, pyridines, and other analogues using S-adenosyl methionine (SAM) as the donor resulting in the production of 1-methylnicotinamide (MNA). NNMT plays a significant role in the regulation of metabolic pathways and is expressed at markedly high levels in several kinds of cancers, neurodegenerative diseases, obesity and diabetes, indicating it is a potential molecular target for therapy. Biovision’s NNMT inhibitor screening kit utilizes SAM as the methyl group donor and nicotinamide as the substrate. NNMT methylates nicotinamide generating S-adenosylhomocysteine (SAH) and 1-methylnicotinamide. The SAH is hydrolyzed by SAH hydrolase to form homocysteine, the free thiol group of which is detected using a Thiol Detecting Probe generating enhanced fluorescence signal that can be measured at Ex/Em = 392/482 nm. In the presence of an NNMT inhibitor, the enzymatic activity is inhibited resulting in decreased fluorescence. This assay kit is a simple, sensitive, and rapid tool to screen potential inhibitors of NNMT.
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