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N-Acetylcysteine Assay Kit (Fluorometric)

for the quantitation of N-Acetylcysteine in biological samples
Catalog #: K2044
$525.00

Product Details

Cat # +Size K2044-100
Size 100 assays
Kit Summary N-Acetylcysteine (NAC) is a precursor of L-cysteine and glutathione biosynthesis. It is a powerful antioxidant and a scavenger of free radicals. It has been commonly used for treating cough, flu, dry eye, lung conditions, paracetamol intoxication etc. Additionally, recent investigations have explored the beneficial effects of NAC in type 2 diabetes and psychiatric disorders. NAC is available as over the counter in tablet, solution as well as intravenous and inhaled preparations. Common side effects of NAC treatment are generally mild and typically resolve on their own once the treatment is stopped. BioVision’s N-Acetylcysteine Assay Kit is a simple and sensitive method for the quantitation of NAC in biological samples. The assay is based on the deacetylation reaction of NAC to generate cysteine. Cysteine is then catalyzed in subsequent reactions thereby producing an intermediate product, which reacts with a fluorogenic probe to form a stable fluorophore measured at Ex/Em = 368/460 nm. The assay is specific with no interference from other thiol-based amino acids.
Detection Method Flourescence (Ex/Em = 368/460 nm)
Species Reactivity All
Applications • Determining the concentration of N-Acetylcysteine in biological samples and pharmaceuticals
Features & Benefits • Sensitive method for the quantitation of NAC in biological samples
• Simple
• The assay is specific with no interference from other thiol-based amino acids
Kit Components • NAC Assay Buffer
• NAC Enzyme Mix I
• NAC Enzyme Mix II
• Reducing Agent
• NAC Blocker
• NAC Probe
• NAC Standard
• 87% TCA Solution
• Neutralization Solution
Storage Conditions -20ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

N-Acetylcysteine (NAC) is a precursor of L-cysteine and glutathione biosynthesis. It is a powerful antioxidant and a scavenger of free radicals. It has been commonly used for treating cough, flu, dry eye, lung conditions, paracetamol intoxication etc. Additionally, recent investigations have explored the beneficial effects of NAC in type 2 diabetes and psychiatric disorders. NAC is available as over the counter in tablet, solution as well as intravenous and inhaled preparations. Common side effects of NAC treatment are generally mild and typically resolve on their own once the treatment is stopped. BioVision’s N-Acetylcysteine Assay Kit is a simple and sensitive method for the quantitation of NAC in biological samples. The assay is based on the deacetylation reaction of NAC to generate cysteine. Cysteine is then catalyzed in subsequent reactions thereby producing an intermediate product, which reacts with a fluorogenic probe to form a stable fluorophore measured at Ex/Em = 368/460 nm. The assay is specific with no interference from other thiol-based amino acids.


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