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Myeloperoxidase (MPO)(Mouse) ELISA Kit

A Sandwich ELISA kit for the quantitative measurement of Myeloperoxidase in mouse serum, plasma, culture supernatants and other biological fluids.
Catalog #: E4580

In stock

$695.00

Product Details

Cat # +Size E4580-100
Size 100 assays
Kit Summary • Detection method- Absorbance (450 nm)
• Species reactivity- Mouse
• Application- quantitative measurement of MPO in mouse serum, plasma, tissue homogenates and other biological fluids.
Detection Method Absorbance (450 nm)
Species Reactivity Mouse
Applications This ELISA kit is used for quantitative measurement of MPO in serum, plasma, tissue homogenates and other biological fluids.
Features & Benefits • Easy, convenient and time-saving method to measure the level of MPO in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.
• Detection Range: 62.5-4000 pg/ml
• Sensitivity: < 37.5 pg/ml
• Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10%
• No significant cross-reactivity or interference was observed.
Kit Components • Micro ELISA Plate
• Lyophilized Standard
• Sample / Standard dilution buffer
• Biotin- detection antibody (Concentrated)
• Antibody dilution buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC dilution buffer
• TMB substrate
• Stop Solution
• Wash buffer (25X)
• Plate sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Myeloperoxidase (MPO) is a peroxidase enzyme most abundantly expressed in neutrophil granulocytes. It is a lysosomal protein stored in the azurophilic granules of the neutrophil. MPO contains a heme pigment which causes its green color in secretions rich in neutrophils, such as pus and some forms of mucus. MPO catalyzes the production of hypochlorous acid (HClO) from hydrogen peroxide (H₂O₂) and chloride anion (Cl-, or the equivalent from a non-chlorine halide). MPO also oxidizes tyrosine to a tyrosyl radical using hydrogen peroxide as an oxidizing agent. BioVision’s Myeloperoxidase ELISA kit is a sandwich ELISA assay for the quantitative measurement of MPO in Mouse serum, plasma and cell culture supernatants. The density of color is proportional to the amount of MPO captured from the samples.


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