Mycoplasma Arginine Deiminase (ADI), Recombinant Protein

Apoptosis inducer & inhibitor of cell proliferation in cultured cells.
Catalog #: P1278 | abID: ab119147

Product Details

abID ab119147
Alternate Name Arginine deiminase, ADI, Arginine dihydrolase, AD, Mycoplasma Arginine Deiminase
Accession # P23793
Source E.coli
Appearance Lyophilized powder or in aqueous solution
Molecular Weight 46.3 kDa
Purity by SDS-PAGE ≥ 97%
Purity by ≥ 97%
Endotoxin Level <0.1 ng/μg of protein (<1EU/μg)
Biological Activity Measured by its ability to induce apoptosis in Jurkat cells using a concentration of 100-150 ng/ml
Reconstitution Instructions Reconstitute in water to a concentration of 0.1–1.0 mg/ml
Amino Acid Sequence SVFDSKFKG IHVYSEIGE LESVLVHEP GREIDYITP ARLDELLFS AILESHDAR KEHKQFVAE LKANDINVV ELIDLVAET YDLASQEAK DKLIEEFLE DSEPVLSEE HKVVVRNFL KAKKTSREL VEIMMAGIT KTDLGIEAD HELIVDPMP NLYFTRDPF ASVGNGVTI HYMRYKVRQ RETLFSRFV FSNHPKLIN TPWYYDPSL KLSIEGGDV FIYNNDTLV VGVSE
Handling Centrifuge the vial prior to opening.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not to be used in humans

Details

Arginine Deiminase (ADI) is a microbial enzyme from Mycoplasma produced in E.coli. It has high affinity to L-arginine and hydrolyzes L-arginine to citrulline and ammonia. Low concentrations of ADI have been shown to inhibit proliferation in certain cultured cells by arresting the cell cycle in G1 and/or S phase. Higher concentrations of ADI lead to subsequent apoptosis. Recombinant Mycoplasma Arginine Deiminase is a 46.3 kDa protein consisting of 409 amino acids


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