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Mutant Alanine Racemase Y354N, Active, Recombinant

Catalyzes the interconversion of L-alanine and D-alanine. Also weakly active on serine.
Catalog #: P1116
SKU-Size Size Price Qty
P1116-200 200 μg
$195.00
P1116-500 500 μg
$685.00
More Sizes Get Quote

Product Details

Alternate Name Alanine Racemase Y354N, alr, dal
Gene Symbol alr
Gene ID 2506170
Accession # P10724
Source E. Coli
Appearance Lyophilized
Physical Form Description Lyophilized form proprietary buffer
Molecular Weight 43 kDa
Purity by SDS-PAGE > 99%
Biological Activity Specific activity is ≥ 50 mU/mg in which one unit enzyme converts 1 µmole of L-serine into D-serine per minute at 37 ºC and pH 7.4 in the presence of pyridoxal phosphate
Reconstitution Instructions Reconstitute enzyme in 50 mM phosphate buffer, pH 7.4.
Handling Centrifuge the vial prior to opening.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not to be used in humans

Details

Mutant Alanine Racemase (Y354N) (mAR-Y354N), a pyridoxal 5-phosphate (PLP) dependent enzyme catalyzes the interconversion of the L-Serine to D-Serine. In WT Alanine Racemase Tyr354 plays a crucial role in defining the strict specificity of AR for alanine, in converting L-Alanine to D-Alanine, which is an important component of the peptidoglycan layer of bacterial cell wall. By mutating the active site Tyr 354 to Asn, the specificity of the enzyme changes and it becomes a racemase with dual specificity for L- Alanine and L-Serine.


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