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MTS Cell Proliferation Colorimetric Assay Kit

based on 4 citations in multiple journalsMTS Cell Proliferation Colorimetric Assay Kit44.1 4
Sensitive, Non-radioactive Assay
Catalog #: K300
SKU-Size Size Price Qty
K300-250 250 assays
K300-500 500 assays
K300-2500 2500 assays
K300-5000 5000 assays
K300-10000 10000 assays
More Sizes Get Quote

Product Details

Kit Summary • Detection- Absorbance (490-500 nm)
• Applications:
- Measurement of cell proliferation in response to growth factors, cytokines, mitogens and nutrients
- Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals
- Assessment of physiological mediators that inhibit cell growth
Detection Method Absorbance (490-500 nm)
Applications Measurement of cell proliferation in response to growth factors, cytokines, mitogens and nutrients.
Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals.
Assessment of physiological mediators that inhibit cell growth.
Features & Benefits • Simple one-step procedure; takes ~ 4 hours
• High-throughput
• The entire assay can be performed directly in a 96-well plate and does not require washing/harvesting/or solubilization steps.
Kit Components • MTS Reagent (in electrocoupling solution)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


BioVision’s MTS Cell Proliferation Assay Kit is a colorimetric method for sensitive quantification of viable cells in proliferation and cytotoxicity assay. The method is based on the reduction of MTS tetrazolium compound by viable cells to generate a colored formazan product that is soluble in cell culture media. This conversion is thought to be carried out by NAD(P)H-dependent dehydrogenase enzymes in metabolically active cells. The formazan dye produced by viable cells can be quantified by measuring the absorbance at 490-500 nm. The assay can be used for the measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients, etc. It can also be used for the analysis of cytotoxic compounds like anticancer drugs and many other toxic agents and pharmaceutical compounds. BioVision’s MTS assay is performed by adding the reagent directly into the cell culture media without the intermittent steps, which are required in the routine MTT assay. In addition, this high-throughput assay requires no washing or solubilization step and can be performed in 96-well microtiter plate.

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What is the difference between MTT and MTS assay?
The formazan salt product of MTS reduction is easily soluble in cell culture medium compared to MTT. This is a faster assay as the reagent is added directly to the cell culture media without the intermittent steps required for MTT assay. Plus there is no washing or solubilization step involved.
Can you use media containing phenol red with this assay?
It's preferred not to use phenol red.
Why do I see high absorbance from media only?
The medium is contaminated with cells/bacteria/yeast (visible under microscope). Use sterile technique for cell plating in biological hood. Use sterile 96-well plate. High background can also occur if media contains ascorbic acid.
Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
What is the shelf life of this kit?
 BioVisions’s assay kits expire 6 months from the date of shipment. Some components of the kit may expire sooner as mentioned in the data sheet
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.
Simon Schimmack, C-reactive protein (CRP) promotes malignant properties in pancreatic neuroendocrine neoplasms. Endocr Connect, June 2019; 31234146.
Yu Yin, N-Myc promotes therapeutic resistance development of neuroendocrine prostate cancer by differentially regulating miR-421/ATM pathway. Mol Cancer, Jan 2019; 30657058.
Sujit Pujhari, Heat shock protein 70 (Hsp70) mediates Zika virus entry, replication, and egress from host cells. Emerg Microbes Infect, Jan 2019;  30866755.
Mao Niu, The role of the ERK1/2 pathway in simvastatin-loaded nanomicelles and simvastatin in regulating the osteogenic effect in MG63 cells. Int J Nanomedicine, Dec 2018; 30584296.
Jesmin Ara, et al., (2018) Hydrogen Water Drinking Exerts Antifatigue Effects in Chronic Forced Swimming Mice via Antioxidative and Anti-Inflammatory Activities, Biomed Res Int, Apr. 2018, 29850492
For more citations of this product click here