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Monoacylglycerol Lipase Inhibitor Screening Kit (Fluorometric)

Industry's most sensitive kit to evaluate potential ECE1 inhibitors
Catalog #: K474

In stock

$495.00

Product Details

Cat # +Size K474-100
Size 100 assays
Kit Summary • Detection method - Fluorescence (Ex/Em = 360/460 nm)
• Applications-
• Screening for inhibitors of human MAGL enzyme
Detection Method Fluorometric (Ex/Em = 360/460 nm)
Species Reactivity Mammalian
Applications • Screening for inhibitors of human MAGL enzyme
Features & Benefits • Simple one-step reaction
• Takes only 1-2 hrs
• Non-radiometric fluorescent detection
• HTP adaptable
Kit Components • MAGL Assay Buffer
• MAGL Substrate (200X)
• MAGL Enzyme (Human Recombinant)
• MAGL Control Inhibitor (JJKK-048)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Monoacylglycerol Lipase (MAGL, E.C. 3.1.1.23) is an important enzyme that regulates endocannabinoid signaling in human physiology. MAGL is a serine hydrolase that generates free fatty acid and glycerol from monoacylglycerol stores. One free fatty acid that is frequently generated by MAGL activity, arachidonic acid, is a precursor for eicosanoids, a family of pro-inflammatory signaling molecules. The glycerol moiety can also serve various purposes as a metabolic building block and energy source. Inhibition or inactivation of MAGL leads to 2-arachidonalglycerol (2-AG) buildup, and this has myriad biological consequences. Desensitization of the endocannabinoid receptors and reduced addiction withdrawal symptoms are one outcome of MAGL inhibition. In addition, the downstream eicosanoid signaling pathway is affected; this pathway plays a role in diverse physiological processes including inflammation, immunity, nociception and blood pressure. As a central node in the regulation of both lipid signaling and energy mobilization, MAGL is of considerable interest as a therapeutic target. BioVision’s Monoacylglycerol Lipase Inhibitor Screening Kit provides a straightforward method to identify inhibitors of human MAGL. In the assay, human recombinant MAGL is used to cleave an MAGL substrate, resulting in a fluorescence increase that is abrogated by the presence of an inhibitor for this enzyme. Specific inhibitors for this enzyme are of great clinical interest due to the central role for MAGL in lipid metabolism and energy storage.


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