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MMP-3 (Human) ELISA Kit

A highly sensitive sandwich ELISA kit for the quantitative measurement of MMP-3

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E5078
$725.00

Product Details

Cat # +Size E5078-100
Size 96 assays
Detection Method Absorbance is measured at 450 nm
Species Reactivity Human
Applications The ELISA kit is used for the quantitative detection of MMP-3 in serum, plasma, tissue homogenates and other biological fluids
Features & Benefits ● Assay Precision; Intra-Assay CV < 8% and Inter-Assay < 10%
● Highly sensitive and specific
● Recovery Rate: 88-101% for serum, 88-103% for EDTA plasma and 87-97% for heparin plasma
● Sensitivity: 0.094 ng/ml
● Detection range: 0.156-10 ng/ml
Kit Components ● ELISA Microplate
● Wash Buffer (25X)
● Plate Sealers
● Lyophilized Standard (10 ng)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody (Concentrated)
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Matrix metalloproteinase-3 (MMP-3) also known as Stromelysin-1 belongs to a family of matrix metalloproteinases. It is involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and tumor metastasis. MMP-3 can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Selective inhibition of MMP-3 and/or targeting MMP generated products could be efficacious for the treatment of prostate to bone metastasis. MMP-3 is abundantly expressed in active rheumatoid synovium, and serum level of MMP-3 is a useful marker not only for the diagnosis of rheumatoid arthritis but also for the evaluation of prognosis in the joint destruction. BioVision’s MMP-3 (Human) ELISA Kit is used for the quantitative detection of MMP-3 in serum, plasma, tissue homogenates and other biological fluids. It is based on the principle of sandwich ELISA. The capture antibody is pre-coated on 96-well plates. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin is added and unbound conjugates are washed away with wash buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of MMP-3 in the sample.


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