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MMP-2 (Human) ELISA Kit

WARNING: This product can expose you to chemicals including TMB, which is [are] known to the State of California to cause cancer.  For more information go to
Catalog #: E4723

Product Details

Cat # +Size E4723-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications • Coefficient of variation is < 10%.
• Detection Range: 0.78—50 ng/ml
• Specificity: This kit recognizes Human MMP-2 in samples. No significant cross-reactivity or interference between Human MMP-2 and analogues was observed.
• A sandwich ELISA kit for in vitro quantitative determination of human MMP-2 concentrations in serum, plasma and other biological fluids.
• Sensitivity: 0.47 ng/ml
Features & Benefits Coefficient of variation is < 10%.
Specificity: This kit recognizes Human MMP-2 in samples. No significant cross-reactivity or interference between Human MMP-2 and analogues was observed.
Detection Range: 0.78—50 ng/ml
Sensitivity: 0.47 ng/ml
Kit Components • Stop Solution
• HRP Conjugate (100x)
• Standard & Sample Diluent
• Wash Buffer (25x)
• Biotinylated Detection Antibody Diluent
• Micro ELISA Plate
• Biotinylated Detection Ab (100x)
• Standard
• Substrate Reagent
• HRP Conjugate Diluent
• Plate Sealer
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


The mammalian Matrix metalloproteinases (MMPs) degrade extracellular matrix in physiological and pathological processes. After cleavage of a single peptide domain of about 20 amino acids, the MMPs are secreted in latent forms. Upon activation, the N-terminal propeptide domain is cleaved to generate the active forms of MMP. MMP-2 (72 kDa type IV collagenase, Gelatinase-A) contains the basic structure of propeptide, catalytic, and hemopexin domains. It is an important proteinase in tissue remodeling. BioVision’s MMP-2 ELISA Kit is based on Sandwich ELISA principle. The micro ELISA plate provided in the kit has been pre-coated with an antibody specific to human MMP-2. On addition of standards or samples to the micro ELISA plate, they react with Biotinylated detection antibody specific for human MMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate, which on the addition of substrate gives blue color. The enzyme-substrate reaction is terminated by the addition of stop solution to turn the reaction yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human MMP-2. The concentration of Human MMP-2 in the samples can be calculated by comparing the OD of the samples to the standard curve.

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