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MMP-12 Activity Assay Kit (Fluorometric)

A homogenous assay that allows the quantification of MMP-12 activity in tissues and sputum samples
Catalog #: K2110
$545.00

Product Details

Cat # +Size K2110-100
Size 100 assays
Kit Summary BioVision’s MMP-12 Activity Assay Kit is a homogenous assay that allows the quantification of MMP-12 activity in tissues and sputum samples.
Detection Method Fluorescence (Ex/Em = 328/418 nm)
Applications Quantification of MMP-12 activity in various biological samples
Features & Benefits The assay is simple, easy, rapid, and has a detection limit of 10 μU MMP-12 activity per well.
Kit Components ● MMP-12 Assay Buffer
● MMP-12 Substrate
● MMP-12 Inhibitor (MMP408)
● Non-Metalloprotease Inhibitor Cocktail
● MMP-12 Positive Control
● MCA Standard
Storage Conditions -20ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

MMP-12, also known as macrophage elastase (EC 3.4.24.65) is a metalloprotease that hydrolyzes the fibrilar connective proteins of the extracellular matrix (ECM) such as elastin and collagen. Like all MMPs, MMP-12 is synthesized as an inactive pro-enzyme, which contains an autoinhibitory domain that interacts with the zinc coordination site in the catalytic domain and must be cleaved for enzymatic activity. MMP-12 is produced by the activated macrophages at sites of inflammation, particularly in pulmonary alveolar tissues. Cleavage of elastin and other ECM proteins play a pivotal role in the pathology of lung diseases, especially chronic obstructive pulmonary disease (COPD) and emphysema. MMP-12 is strongly expressed in chronic smokers with COPD. MMP-12 activity has also been implicated in the development of atherosclerosis and ischemic stroke due to catabolism of ECM surrounding fibrotic plaques. BioVision’s MMP-12 Activity Assay Kit is a homogenous assay that allows the quantification of MMP-12 activity in tissues and sputum samples. The assay utilizes a synthetic peptide substrate bearing both a fluorophore and a fluorescence quencher. Upon cleavage by active MMP-12, the fluorophore-bearing peptide fragment is unquenched to produce a bright fluorescent signal measured at Ex/Em = 328/418 nm. A selective MMP-12 inhibitor is included in the kit for determination of MMP-12 activity in heterogeneous biological samples, where other MMP isozymes may contribute to peptide substrate cleavage. The inhibitor displays 100-fold selectivity for MMP-12 over other MMPs, ensuring targeted inhibition. A general protease inhibitor cocktail that inhibits non-metalloproteases in tissue homogenates is also included. The assay is simple, easy, rapid, and has a detection limit of 10 μU MMP-12 activity per well.


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