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MLKL (Human) ELISA Kit

A Sandwich ELISA kit for quantitative measurement of MLKL in human serum, plasma, tissue lysates, and other biological fluids
Catalog #: E5002
$810.00

Product Details

Cat # +Size E5002-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications Sandwich ELISA kit for quantitative measurement of MLKL in serum, plasma, cell and tissue lysates, and other biological fluids
Features & Benefits ● Detection range: 0.313 – 20 ng/ml
● Recovery range: 85 - 105% for normal human serum and plasma samples
● Assay Precision: Intra-Assay CV and Inter-Assay CV < 10%
● This Sandwich ELISA is highly sensitive and highly specific for the detection of MLKL in human samples. There is no significant cross-reactivity or interference between MLKL and analogues
● Sensitivity: 0.188 ng/ml
Kit Components ● Micro ELISA plate
● Wash Buffer (25X)
● Plate sealers
● Standard (Lyophilized) (20 ng)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate Solution
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Mixed lineage kinase domain-like pseudokinase (MLKL) is a terminal effector protein of necroptosis, an alternate form of cell death that mimics the characteristics of necrosis and apoptosis. MLKL possesses a pseudokinase domain at the C-terminus, a two-helix linker, and a four-helix bundle at the N-terminus. RIPK3 phosphorylates MLKL at threonine 357/serine 358 in this pseudokinase domain. Upon phosphorylation, MLKL undergoes a conformational change and results in the exposure of the four-helix bundle domain (4HB). Simultaneously, TAM (Tyro3, Axl, and Mer) kinase phosphorylates MLKL at tyrosine 376 and induces its oligomerization. After oligomerization, MLKL associates with chaperone proteins HSP90 and HSP70 that assist in the translocation to the plasma membrane. MLKL disrupts the integrity of the plasma membrane and subsequently causes necroptosis. Since it is the only known substrate of RIPK3 that can efficiently induce necroptosis, MLKL may be considered a promising target for anti-inflammation and anti-cancer therapies. BioVision’s MLKL (Human) ELISA kit quantitatively measures MLKL in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. HRP-Streptavidin is added to the plate, and post-incubation any unattached conjugates are washed off by Wash Buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of MLKL in the sample or standard.


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