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Product Details
| Cat # +Size | K285-50 |
|---|---|
| Size | 50 assays |
| Applications | Optimized for compatibility with immunoprecipitation (IP and co-IP) and pull-down using tagged proteins. Gentle formulation for maintenance of stable mitochondrial complexes. Compatible with SDS PAGE, 2D gel, Blue-Native gel, Mass spectrometry. Functional Assays and enzymatic assays. |
| Features & Benefits | • Simple & convenient protocol • The Kit provides unique formulation of ready-to-use buffers & reagents for IP/Co-IP/Pull down of mitochondrial proteins or complexes. |
| Kit Components | • Mitochondria Protein IP Buffer • Wash Buffer (5X) • Protease Inhibitor Cocktail • 10% n-Dodecyl-beta-D-maltoside • 10% Triton X-100 • 10% Digitonin |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Mitochondria are the power house of the cells and play an essential role in energy production. Damage to the mitochondria activates signaling pathways that induce apoptosis. Mitochondria also regulate and mediate transport of the metabolites and ions needed for oxidative phosphorylation and maintenance of membrane potential for ATP synthesis. Mitochondrial dysfunction leads to several disorders like cardiac dysfunction, diabetes, aging and neurological disorder, mainly caused by mutations in mitochondrial DNA or in nuclear genes that code for mitochondrial components. Thus, mitochondria have several different functions in the cell. BioVision ready to use mitochondria Protein IP Buffer is optimized for immunoprecipitation (IP and co-IP) using mitochondria and mitochondrial extracts. The buffer is a gentle formulation, which maintains the stability of mitochondrial complexes. The Mitochondrial Protein IP kit is provided with different choices of detergents like n-Dodecyl-beta-D-maltoside, Triton X-100 and digitonin to achieve different stringency conditions for protein-protein interaction studies. Triton X-100 is the most commonly used detergent especially for membrane protein solubilization. However, in case of fragile complexes digitonin or n-Dodecyl-beta-D-maltoside is the choice of detergents.
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Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.
