Mitochondrial Complex III Activity Assay Kit (ab287844)
Overview
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Product name
Mitochondrial Complex III Activity Assay Kit -
Detection method
Colorimetric -
Sample type
Purified mitochondria -
Assay type
Quantitative -
Sensitivity
10 mU/ml -
Assay duration
Multiple steps standard assay -
Product overview
This Mitochondrial Complex III Activity Assay kit (ab287844) is based on this reduction of cytochrome c through the activity of Complex III. The absorbance of reduced cytochrome c can be measured at 550 nm. It is a fast and reliable method to determine the activity of complex III in isolated mitochondria. The kit can detect as low as 10 mU/ml and is linear up to 30 mU/ml.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K520 Mitochondrial Complex III Activity Assay Kit. K520-100 is the same size as the 100 test size of ab287844.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Antimycin A 1 x 220µl 96-Well Half Area Plate 1 unit Complex III Assay Buffer 1 x 25ml DTT IV 1 x 1ml Oxidized Cytochrome c 4 vials -
Research areas
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Relevance
BCS1L (BCS1-like) is a chaperone necessary for the assembly of mitochondrial respiratory chain complex III. The protein does not contain a mitochondrial targeting sequence but experimental studies confirm that it is imported into mitochondria. Mutations in this gene are associated with mitochondrial complex III deficiency and the GRACILE syndrome. Two alternatively spliced transcripts encoding the same protein have been described. -
Cellular localization
Mitochondrial. Mitochondrion inner membrane. Single-pass membrane protein. -
Alternative names
- BCS
- BCS 1
- BCS 1L
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab287844 has not yet been referenced specifically in any publications.