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Mitochondrial Complex I Activity Colorimetric Assay Kit

Catalog #: K968
$595.00

Product Details

Cat # +Size K968-100
Size 100 assays
Detection Method Colorimetric (Absorbance 600 nm)
Species Reactivity Multiple species
Applications Measurement of Complex I activity in isolated mitochondria
Features & Benefits • Simple, rapid & convenient assay to measure Mitochondrial Complex I
• Includes Inhibitor (Rotenone)
Kit Components • Complex I Assay Buffer
• NADH • Decylubiquinone
• Complex I Dye
• Complex I Inhibitor Rotenone
• Clear 96-well half area plate
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Mitochondrial Complex I or NADH:ubiquinone oxidoreductase (EC 1.6.5.3) is the first and the largest complex of the Electron Transport Chain located in the mitochondrial membrane. It oxidizes NADH to NAD+ and transfers an electron to ubiquinone (also present in the inner mitochondrial membrane) converting it to ubiquinol. During this process, it transports protons across the inner mitochondrial membrane, helping to develop an electrochemical gradient. This process is very important for cellular respiration and adverse effects on Complex I activity can compromise mitochondrial respiration, which further leads to cellular stress. BioVision’s Mitochondrial Complex I assay kit is a fast and reliable method to determine the activity of complex I in isolated mitochondria. It is useful for respiration studies in isolated mitochondria and may be used to study effects of toxicants, drugs and other environmental conditions on mitochondrial complex I activity. This kit uses decylubiquinone, an analog of ubiquinone, as an electron acceptor that gets converted to decylubiquinol through the catalytic activity of Complex I. The Complex I dye that absorbs light at 600 nm in its oxidized form is used as a terminal electron acceptor that accepts electrons from decylubiquinol. Complex I activity is determined colorimetrically by recording the change in absorbance of reduced Complex I dye at 600 nm. Specific Complex I activity is obtained by subtracting the activity in presence of Complex I inhibitor rotenone from total activity. This kit can detect as low as 0.1 mU / well and is linear up to 7 mU / well.


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Mohamad-Reza Aghanoori, Insulin-like growth factor-1 activates AMPK to augment mitochondrial function and correct neuronal metabolism in sensory neurons in type 1 diabetes. Mol Metab, Feb 2019;  30545741.
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