Mitochondrial Complex I Activity Colorimetric Assay Kit (ab287847)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate (12 x 8 well strips)
- Sample type: Purified mitochondria
- Sensitivity: 0.1 mU/well
Overview
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Product name
Mitochondrial Complex I Activity Colorimetric Assay Kit -
Detection method
Colorimetric -
Sample type
Purified mitochondria -
Assay type
Enzyme activity -
Sensitivity
0.1 mU/well -
Assay duration
Multiple steps standard assay -
Product overview
The Mitochondrial Complex I assay kit ab287847 is a fast and reliable method to determine the activity of complex I in isolated mitochondria. It is useful for respiration studies in isolated mitochondria and may be used to study effects of toxicants, drugs and other environmental conditions on mitochondrial complex I activity. This kit uses decylubiquinone, an analog of ubiquinone, as an electron acceptor that gets converted to decylubiquinol through the catalytic activity of Complex I. The Complex I dye that absorbs light at 600 nm in its oxidized form is used as a terminal electron acceptor that accepts electrons from decylubiquinol. Complex I activity is determined colorimetrically by recording the change in absorbance of reduced Complex I dye at 600 nm. Specific Complex I activity is obtained by subtracting the activity in presence of Complex I inhibitor rotenone from total activity. This kit can detect as low as 0.1 mU / well and is linear up to 7 mU / well.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K968 Mitochondrial Complex I Activity Colorimetric Assay Kit. K968-100 is the same size as the 96 test size of ab287847.
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Tested applications
Suitable for: Functional Studiesmore details -
Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 96 tests 100X NADH Stock 1 vial 96-Well Half Area Plate 1 unit Assay Buffer XLVII 1 x 25ml Complex I Dye 1 vial Complex I Inhibitor 1 x 100µl Decylubiquinone 1 vial
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab287847 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Functional Studies |
Use at an assay dependent concentration.
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Notes |
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Functional Studies
Use at an assay dependent concentration. |
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab287847 has not yet been referenced specifically in any publications.