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Mitochondrial ATP synthase Activity Assay Kit (Colorimetric)

A simple, plate-based colorimetric assay for measuring ATP synthase in isolated mitochondria
Catalog #: K2112
$595.00

Product Details

Cat # +Size K2112-100
Size 100 assays
Kit Summary BioVision’s Mitochondrial ATP Synthase Activity Assay Kit is a simple, plate-based colorimetric assay for measuring ATP synthase in isolated mitochondrial samples.
Detection Method Absorbance at 340 nm
Species Reactivity Mammalian
Applications Measurement of Mitochondrial ATP synthase enzymatic activity in isolated mitochondria
Features & Benefits The assay provides a simple, rapid, and sensitive method to measure ATP synthase activity in isolated mitochondria
Kit Components ● ATP Synthase Assay Buffer
● ATP
● ATP Synthase Converter
● NADH
● ATP Synthase Enzyme Mix
● Oligomycin
● Purified Mitochondria
● 96-well Half Area Clear Plate
Storage Conditions Multiple Temperatures
Shipping Conditions Dry Ice
USAGE For Research Use Only! Not For Use in Humans.

Details

Mitochondrial ATP synthase (EC 3.6.3.14), also known as mitochondrial complex V or F1F0-ATP synthase is a multi-subunit membrane-bound enzyme of mitochondria that utilizes the proton electrochemical gradient generated by the respiratory chain for ATP synthesis. It consists of two domains, an inner membrane embedded domain (F0) and a membrane extrinsic domain (F1), which is also the catalytic core of the enzyme. In addition to its critical role in ATP synthesis, ATP synthase regulates the flux of oxidative phosphorylation, mitochondrial signaling by reactive oxygen species, cell death etc. Defects in ATP synthase have been associated with human diseases including neuromuscular disorders, heart failure etc. Thus, ATP synthase may be used as a therapeutic drug target in the treatment of many diseases. BioVision’s Mitochondrial ATP Synthase Activity Assay Kit is a simple, plate-based colorimetric assay for measuring ATP synthase in isolated mitochondrial samples. In this assay, ATP Synthase hydrolyzes ATP to ADP. In the subsequent steps, ADP together with ATP synthase converter and enzyme mix oxidizes NADH to NAD+, which is monitored via a decrease in absorbance at 340 nm. A specific ATP synthase inhibitor, oligomycin is also included in the kit. Sample specific ATP synthase activity is obtained by measuring the sample ATP synthase activity in the presence and absence of oligomycin. The assay provides a simple, rapid, and sensitive method to measure ATP synthase activity in isolated mitochondria.


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