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Minimal Inhibitory Concentration Kit

For Gram Negative Rods

WARNING: This product can expose you to chemicals including Gentamicin sulfate, which is [are] known to the State of California to cause birth defects or other reproductive harm.  For more information go to  www.P65Warnings.ca.gov.
Catalog #: K195
SKU-Size Size Price Qty
K195-500 500 assays
$395.00
K195-2500 2500 assays
$595.00
More Sizes Get Quote

Product Details

Kit Summary BioVision’s Minimal Inhibitory Concentration (MIC) kit, testing for Gram Negative Rods (GNR) is a broth microdilution assay that is simple yet sensitive for the quantification of any antimicrobial agent against a given bacterial isolate. The antibiotic efficacy is quantified using a Water Soluble Tetrazolium salt (WST) which changes color reflecting the viability of the bacteria as a response to antibiotic treatment. The test is performed in a microplate with bacteria-inoculated-Luria Bertani (LB) broth and serial dilutions of antimicrobial agents can be added. Our kit includes four antibiotics often prescribed for infections caused by gram negative rods. They are: Ampicillin Sodium, Chloramphenicol, Enrofloxacin, and Gentamicin Sulfate. Vancomycin Hydrochloride, an antibiotic effective in the treatment of gram positive cocci, is included as a negative control. The Antibiotic Susceptibility Limits of these antibiotics have been evaluated and correlated with published standards (Andrews, 2001) for Minimum Inhibitory Concentrations.
Detection Method Absorbance, OD 460 nm
Species Reactivity Gram Negative Rods, E. coli or other gram negative bacteria
Applications • Quick counting and Time-Course assay protocols
• Determination of antibiotic susceptibility, MIC, resistance and viability in E. coli or other gram negative bacteria.
Features & Benefits • Broth Microdilution Assay
• Quantification of any antimicrobial agent against a given bacterial isolate
• Sensitive
• Simple
Kit Components • Vancomycin Hydrochloride (50 mg/ml)
• Gentimicin Sulfate (50 mg/ml)
• Chloramphenicol (34 mg/ml)
• AB Buffer
• Ampicillin Sodium (100 mg/ml)
• WST Reagent
• Enrofloxacin (3.7 mg/ml)
• Electrocoupling Solution (ECS)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Testing antibiotic susceptibility in bacterial isolates enables the detection of drug resistance in common bacterial pathogens. This ensures susceptibility to selected pharmaceutical interventions for specific pathogens classified as gram negative rods. The broth microdilution, a well-known method in microbiology is flexible and provides quantitative and semi-quantitative results to monitor resistance amongst bacterial pathogens. It is called microdilution because it involves the use of small volumes of broth dispensed in sterile, plastic microdilution trays (~0.1 ml of broth). BioVision’s Minimal Inhibitory Concentration (MIC) kit, testing for Gram Negative Rods (GNR) is a broth microdilution assay that is simple yet sensitive for the quantification of any antimicrobial agent against a given bacterial isolate. The antibiotic efficacy is quantified using a Water Soluble Tetrazolium salt (WST) which changes color reflecting the viability of the bacteria as a response to antibiotic treatment. The test is performed in a microplate with bacteria-inoculated-Luria Bertani (LB) broth and serial dilutions of antimicrobial agents can be added. Our kit includes four antibiotics often prescribed for infections caused by gram negative rods. They are: Ampicillin Sodium, Chloramphenicol, Enrofloxacin, and Gentamicin Sulfate. Vancomycin Hydrochloride, an antibiotic effective in the treatment of gram positive cocci, is included as a negative control. The Antibiotic Susceptibility Limits of these antibiotics have been evaluated and correlated with published standards (Andrews, 2001) for Minimum Inhibitory Concentrations.


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