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Methionine Assay Kit (Fluorometric)

The first kit in the market detecting free methionine in a variety of biological samples. Highly sensitive.
Catalog #: K442
$565.00

Product Details

Cat # +Size K442-100
Size 100 assays
Kit Summary • Detection method- Fluorescence (Ex/Em = 535/587 nm)
• Species reactivity- Eurkaryotes
• Applications- Estimation of Methionine in various biological samples.
• Detection limit/sensitivity: Can detect as little as 0.5 µM of Methionine
Detection Method Fluorescence (Ex/Em = 535/587 nm)
Species Reactivity Eukaryotes
Applications Estimation of Methionine in various biological samples
Features & Benefits • Simple, sensitive and HTP adaptable Protocol
• Specific for Methionine without interference from other thiol based amino acids
• This kit can detect as little as 0.5 µM of Methionine in a variety of biological samples
Kit Components • Met Assay Buffer
• Met Buffer Supplement
• Met Enzyme Mix I
• Met Enzyme Mix II
• Met Developer
• Met Probe
• Sample Clean-Up Mix
• Met Standard (10 mM)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Methionine (Met) is one of the twenty proteogenic amino acids encoded by the genetic code, and one of nine essential amino acids, meaning it cannot be synthesized in vivo and must be obtained from dietary sources. Met is one of two sulfur-containing amino acids (the other being cysteine) and can serve as a precursor in cysteine biosynthesis. S-adenosylmethionine, another product of Met metabolism, is an important co-factor and methyl donor in a number of biological reactions such as methylation and polyamine synthesis. Met is also an intermediate species in the synthesis of phospholipids such as lecithin, and other small precursor molecules including taurine, an amino-sulfonic acid. Oxidation of Met generates methionine sulfoxide, a species whose concentration tends to increase with age and is known to cause protein mis-folding and loss-of-function of numerous enzymes. This version of the amino acid can be restored back to methionine by another enzyme, methionine sulfoxide reductase. Biologically, Met levels can affect important events such as angiogenesis, protein synthesis, and cartilage production. BioVision’s Methionine Assay Kit utilizes an enzymatic mechanism by which metabolism of methionine is coupled with the stoichiometric generation of hydrogen peroxide. A probe generates fluorometric signal that can be quantified at Ex/Em: 535/587 nm. The assay shows greater than 10-fold specificity for methionine over cysteine and greater than 40-fold selectivity over other standard and non-standard amino acids. After utilization of a proprietary Sample Clean-Up Mix, the method is suitable for use in blood samples, and can detect concentrations as low as 0.5 µM, or 25 pmole Methionine.


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Can you please provide me the protocol for preparation of cell lysate?
Please start with 1x106 cells. Add 100-200 µl of Met assay buffer (K442-100-1). Homogenize using a dounce homogenizer or by gentle vortexing and make sure the cells are lysed. Centrifuge at 13,000 g for 15 min at 4°C. Take the supernatant and filter the samples using a 10 kDa spin column (10000 x g, 4 °C, 10 min; BioVision Cat#1997). Retain the ultra-filtrate for the assay.