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Methanol Assay Kit (Colorimetric)

Market's first and only kit for the enzymatic detection of Methanol in biological samples
Catalog #: K898

Product Details

Cat # +Size K898-100
Size 100 assays
Kit Summary • Detection method-Absorbance (450 nm)
• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other samples
• Species reactivity- Mammalian /others
• Application- The kit detects 500 ppm Methanol
Detection Method Absorbance (450 nm)
Species Reactivity Mammalian /others
Applications Determination of methanol in biological samples, anaerobic bacterial cultures, agricultural products and food products
Features & Benefits • Simple procedure; takes ~40 minutes - 1 hour
• Fast and convenient
• Kit contains the necessary reagents for accurate and specific measurement of Methanol in biological samples
Kit Components • Methanol Assay Buffer
• Methanol Developer
• Methanol Enzyme Mix
• Methanol Probe
• Pure Methanol Stock (24.7 M)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Methanol is the simplest alcohol, consisting exclusively of a methyl group and a hydroxyl moiety. It is both an important industrial molecule (solvent, fuel, building block for chemical synthesis) and a biological metabolite; many bacteria generate methanol as a result of anaerobic metabolism. In humans, ingestion of large quantities of methanol is toxic and suppresses the nervous system; this is termed ‘methanol poisoning’. As such, methanol is frequently used as an additive in industrial alcohols to prevent human consumption. The toxicity of methanol is primarily due to the biological product of its metabolism, formaldehyde, which is further metabolized into formic acid. At lower concentrations, methanol poisoning can cause loss of coordination and discomfort, and at higher concentrations will lead to kidney failure, blindness, and even death. In the gut, microbial breakdown of pectin-rich foods produces small amounts of methanol. In addition, some bacteria are capable of metabolizing methane gas, generating methanol, and so monitoring methanol concentration is also relevant for industrial purposes and renewable energy research. BioVision’s Methanol Assay Kit utilizes an enzymatic mechanism by which conversion of methanol is correlated stoichiometrically with generation of a colorimetric signal that can be quantified at 450 nm. The assay shows greater than 100-fold specificity for methanol over ethanol. The method is suitable for use in a range of biological and consumable samples, and can detect as little as 500 pmol methanol.

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