Melatonin ELISA Kit (ab285251)
Key features and details
- Sensitivity: 4.688 pg/ml
- Range: 7.813 pg/ml - 500 pg/ml
- Sample type: Other biological fluids, Plasma, Serum, Tissue Homogenate
- Detection method: Colorimetric
- Assay type: Competitive
Overview
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Product name
Melatonin ELISA Kit
See all Melatonin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Melatonin < 8% Inter-assay Sample n Mean SD CV% Melatonin < 10% -
Sample type
Serum, Plasma, Other biological fluids, Tissue Homogenate -
Assay type
Competitive -
Sensitivity
< 4.688 pg/ml -
Range
7.813 pg/ml - 500 pg/ml -
Assay duration
Multiple steps standard assay -
Product overview
The Melatonin ELISA kit (ab285251, E4630) is a competitive ELISA assay for the quantitative measurement of melatonin in serum, plasma and cell culture supernatants. The density of color is proportional to the amount of melatonin captured from the samples.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 96 tests 25X Wash buffer 1 x 30ml Antibody dilution buffer 1 x 10ml Biotin/Detection antibody (Concentrated) 1 x 60µl HRP-Streptavidin Conjugate (SABC) 1 x 120µl Lyophilized Standard 1 x 2 vials Micro ELISA Plate 1 unit Plate Sealer 1 x 5 units SABC dilution buffer 1 x 10ml Sample / Standard Dilution Buffer 1 x 20ml Stop Solution 1 x 10ml TMB Substrate 1 x 10ml -
Research areas
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Relevance
Melatonin, the principal hormone of the pineal gland, is also produced by the retina, lens and GI tract. It is naturally synthesized from the amino acid tryptophan (via synthesis of serotonin) by the enzyme 5-hydroxyindole-O-methyltransferase. Production of melatonin by the pineal gland is under the influence of the suprachiasmatic nucleus of the hypothalamus (SCN -the site of a circadian clock) which receives information from the retina about the daily pattern of light and darkness. Melatonin can alter the timing of mammalian circadian rhythms, as well as regulate the reproductive alterations that occur in response to changes in day length in seasonally breeding mammals. Melatonin is also an extremely powerful antioxidant, with a particular role in the protection of nuclear and mitochondrial DNA. Therapeutically there may be many potential uses for melatonin such as in the treatment of various forms of some forms of depression, cancer, HIV, plus other viral diseases. Currently, Melatonin is a popular therapy for jet-lag and disturbances of sleep. -
Cellular localization
Secreted -
Alternative names
- 5 methoxy N acetyltryptamine
- Melatonin
- N acetyl 5 methoxytryptamine
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab285251 has not yet been referenced specifically in any publications.