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Melatonin ELISA Kit

A competitive ELISA kit for the quantitative measurement of melatonin in serum, plasma, tissue homogenates and other biological fluids

WARNING: This product can expose you to chemicals including TMB, which is [are] known to the State of California to cause cancer.  For more information go to
Catalog #: E4630

Product Details

Cat # +Size E4630-100
Size 100 assays
Kit Summary • Detection method- Absorbance (450 nm)
• Species reactivity- Universal
• Application- quantitative measurement of melatonin in serum, plasma, tissue homogenates and other biological fluids.
Detection Method Absorbance (450 nm)
Species Reactivity Universal
Applications This ELISA kit is used for quantitative measurement of melatonin in serum, plasma, tissue homogenates and other biological fluids.
Features & Benefits • Easy, convenient and time-saving method to measure the level of melatonin in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.
• Detection Range: 7.813-500 pg/ml
• Sensitivity: < 4.688 pg/ml
• Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10%
• No significant cross-reactivity or interference was observed.
Kit Components • Micro ELISA Plate
• Lyophilized Standard
• Sample / Standard dilution buffer
• Biotin- detection antibody (Concentrated)
• Antibody dilution buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC dilution buffer
• TMB substrate
• Stop Solution
• Wash buffer (25X)
• Plate sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Melatonin is a hormone that is produced by the pineal gland in humans and animals and regulates sleep and wakefulness. Melatonin is also produced in plants where it functions as a first line of defense against oxidative stress. Melatonin is involved in the entrainment (synchronization) of the circadian rhythms including sleep-wake timing, blood pressure regulation, seasonal reproduction, and many others. BioVision’s melatonin ELISA kit is a competitive ELISA assay for the quantitative measurement of melatonin in serum, plasma and cell culture supernatants. The density of color is proportional to the amount of melatonin captured from the samples.

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What species was used to produce the primary antibody (rabbit, goat, sheep, mouse)?
The primary antibody is a rabbit polyclonal antibody.
What is the nature of the labelled melatonin (i.e 125I-2-iodomelatonin, iodinated melatonin analogue, melatonin conjugated to biotin or an enzyme)?
Melatonin is labelled with biotin.
What is the source and purity of the melatonin used for the standards? There is to my knowledge currently no melatonin reference standard
Purity of melotonin is >= 98% by TLC.