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MCP-3 (Human) ELISA Kit

A Sandwich ELISA kit for the quantitative measurement of human MCP-3 in human Serum, plasma and other biological fluids.
Catalog #: E4836

Product Details

Cat # +Size E4836-100
Size 96 assays
Detection Method Sandwich ELISA, Absorbance (450 nm)
Species Reactivity Human
Applications A Sandwich ELISA kit for the quantitative measurement of human MCP-3 in human Serum, plasma and other biological fluids.
Features & Benefits • Sensitivity: 9.38 pg/mL
• Precision: Detection Range: 15.63-1000 pg/mL
• Detection Range: 15.63-1000 pg/mL
• Specificity: This kit recognizes Human MCP-3 in samples. No Significant cross-reactivity or interference between Human MCP-3 and analogues was observed.
Kit Components • Micro ELISA Plate
• Reference Standard
• Biotinylated Detection Ab (100x)
• HRP Conjugate (100x)
• Reference Standard & Sample Diluent
• Biotinylated Detection Antibody Diluent
• HRP Conjugate Diluent
• Wash Buffer (25X)
• Substrate Reagent
• Stop Solution
• Plate Sealer
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Macrophage/Monocyte Chemotactic Protein-3 (MCP-3) plays an important role in the inflammatory response of blood monocytes, tissue macrophages, and eosinophils. MCP-3 is a chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. It is also known to augments monocyte anti-tumor activity and induces the release of gelatinase B. BioVision’s MCP-3 (Human) ELISA Kit is based on the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MCP-3. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Human MCP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of MCP-3 bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human MCP-3. The concentration of Human MCP-3 in the samples can be calculated by comparing the OD of the samples to the standard curve.

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