Call 408.493.1800 | Fax 408.493.1801 | Toll Free 800.891.9699 (US Only) | Email: [email protected]

New

MCP-1 (Rat) ELISA Kit

A Sandwich ELISA kit for the quantitative measurement of rat MCP-1 in Serum, plasma and other biological fluids
Catalog #: E4835
$765.00

Product Details

Cat # +Size E4835-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Rat
Applications in vitro quantitative measurement of rat MCP-1 in Serum, plasma and other biological fluids
Features & Benefits • Specificity: This kit recognizes Rat MCP-1 in samples. No significant crossreactivity or interference between Rat MCP-1 and analogues was observed
• Precision: Coefficient of variation is < 10%
• Sensitivity: 0.10 ng/mL
• Detection Range: 0.16-10 ng/mL
Kit Components • Micro ELISA Plate
• Stop Solution
• Plate Sealer
• Reference Standard
• Biotinylated Detection Ab (100x)
• HRP Conjugate (100x)
• Reference Standard & Sample Diluent
• Biotinylated Detection Antibody Diluent
• HRP Conjugate Diluent
• Wash Buffer (25x)
• Substrate Reagent
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

MCP-1 acts as a ligand for C-C chemokine receptor CCR2. It signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. It exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. It is also involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. BioVision’s MCP-1 (Rat) ELISA Kit is based on the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MCP-1. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Rat MCP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of MCP-1 bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Rat MCP-1. The concentration of Rat MCP-1 in the samples can be calculated by comparing the OD of the samples to the standard curve.


Why buy BioVision Products?
Innovation
Affordability
Global Presence
Technical Support
BioVision aims to provide our customers innovative tools for accelerating drug discovery and biological research. BioVision offers >8,000 products including the most comprehensive array of assay kits for key targets in Metabolic pathways.
BioVision is committed to providing the highest quality products at a competitive price.
We have a broad network of global distributors who are ready to address your research needs and ensure fast delivery.
Our highly trained Technical Support team provides comprehensive product support and is dedicated to resolving your issues quickly and efficiently.