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MBP (Human) ELISA Kit

A Sandwich ELISA kit for quantitative measurement of Myelin Basic Protein (MBP) in human serum, plasma, tissue lysates, and other biological fluids
Catalog #: E4974
$795.00

Product Details

Cat # +Size E4974-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications Sandwich ELISA kit to quantitatively measure MBP in human serum, plasma, tissue lysate, and other biological fluids
Features & Benefits ● Sensitivity: 0.094 ng/ml
● Recovery range: 85 - 105% for normal human serum and plasma samples
● Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%
● This Sandwich ELISA is highly sensitive and highly specific for the detection of MBP in human samples. There is no significant cross-reactivity or interference between MBP and analogues
● Detection range: 0.156 – 10 ng/ml
Kit Components ● Micro ELISA plate
● Wash Buffer (25X)
● Plate Sealers
● Standard (Lyophilized) (10 ng)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate Solution
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Myelin basic protein (MBP) is a key structural protein of the myelin sheath of oligodendrocytes and Schwann cells in the central nervous system. The 169 amino acid peptide makes up to 30% of the myelin sheath. MBP interacts with multiple cytoskeletal proteins such as actin, tubulin, and calmodulin and regulates their bundling and polymerization. MBP can also bind to signaling molecules that are important for differentiation and myelination. It plays an important role in demyelinating diseases such as multiple sclerosis. MBP undergoes deimination, a type of post-translational modification wherein arginine residues are converted to citrulline. Increased deimination of MBP positively correlates with Multiple sclerosis. BioVision’s MBP (Human) ELISA kit is based on the Sandwich principle to quantitatively measure the concentration of MBP in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off by Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is directly proportional to the concentration of MBP in the sample or standard.


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