Product Details
| Detection Method | Purification of plasmids using magnetic beads in a 96-well plate |
|---|---|
| Applications | Parallel purification of plasmids from 96 samples |
| Features & Benefits | • Rapid, easy and convenient
• Many downstream applications such as DNA Sequencing, Restriction Enzyme digestion etc. • Highly pure DNA, high yield • Highly efficient Magnetic particles |
| Kit Components | • Sealing film
• Buffer A1 • *RNaseA • Buffer B1 • Buffer N1 • 96-well Lysate Clearance Plate • MagPure Particle Solution • **DNA Wash Buffer • Elution Buffer • 96-well Binding Plate (500 μl) |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
BioVision's MagPure™ 96-well Plasmid Purification Kit combines the efficiency of magnetic bead technology with alkaline-SDS lysis of bacterial cells to purify high quality plasmid DNA. The kit is used to extract and purify plasmids using a 96-well Lysate Clearance Plate and magnetic particles. The cleared lysate obtained using the 96-well clearance plate in the first step is further purified using magnetic beads. The proprietary magnetic particles bind to DNA or RNA avidly, but reversibly under optimal conditions. The new 96-well Lysate Clearance Plate removes the need of time-consuming centrifugation to clear bacterial alkaline lysates. Proteins and other contaminants are removed and the nucleic acids can then be easily eluted with deionized water or low salt buffer. The purified plasmid can be used directly for automated fluorescent DNA sequencing and other standard molecular biology techniques such as restriction enzyme digestion. 1 ml of overnight culture in LB medium typically yields 10-15 μg high-copy plasmid DNA.
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