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Magnesium Colorimetric Assay Kit

based on 1 citations in multiple journalsMagnesium Colorimetric Assay Kit14.1 4
Catalog #: K385

In stock

$345.00

Product Details

Cat # +Size K385-100
Size 100 assays
Detection Method Absorbance (450 nm)
Species Reactivity Mammalian
Applications The linear range of the assay is 2-15 nmoles with detection sensitivity~ 40 µM.
Features & Benefits • Simple procedure; takes ~ 3 hours
• Fast and convenient
• The assay is sensitive and stable
• The TUNEL-based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections.
Kit Components • Magnesium Assay Buffer
• Magnesium Developer
• Magnesium Enzyme Mix
• Magnesium Standard (150 nmol/µl)
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Magnesium is the 11th most abundant element by mass in the human body. Mg²ᶧ is essential to all • Living cells where it plays an important role in facilitating the processing of biological polyphosphates like ATP, DNA, RNA and enzyme functions. Mg²ᶧ is the metallic ion at the center of chlorophyll, and a common additive to fertilizers. Mg²ᶧ compounds are used as laxatives, antacids, and used to stabilize abnormal nerve excitation and blood vessel spasm i.e., eclampsia. The BioVision Magnesium Assay Kit provides a simple sensitive means of quantitating magnesium in a variety of biological samples. The kit takes advantage of the specific requirement of glycerol kinase for Mg²ᶧ. An enzyme linked reaction leads to formation of an intensely colored (λmax = 450nm) product whose formation is proportional to Mg²ᶧ concentration. The linear range of the assay is 2-15 nmoles with detection sensitivity~ 40 µM.


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Our customer is looking for a test kit for the quantitative determination of magnesium in EGTA-masked samples and photometric measurement should be made as quickly as possible. His sample is some DNA, some RNA and some enzymes and proteins. Thus, this customer would like to know if this kit is suitable for?
Yes, this assay kit would most definitely be suitable for any biological samples they are planning on using. When you say DNA/RNA samples, are these purified samples. In such case does the client know that there will be free Mg, (other than what is present in the solvent in this solution) to be assayed? I just want to be sure of this, before they start using the kit.
(1) I would like to know that after “Magnesium Standard” was once thawed, the aliquot of “Magnesium Standard” can be stored at -20? again until the next assay.(2) I would like to know wheather the 96 well plates used in this assay should be flat bottom or U bottom.(3) Should this assay be done in duplicate?
Yes, once thawed, the standard can be stored in aliquots at -20°C.You can use a flat bottom plate for the assay.Doing the assay in triplicates would be best, but if that is not possible, duplicates are required.
I bought the magnesium assay kit (#K385-100) from Biovision last month and used it to measure Mg++ concentration for my acidified biological samples. All these samples are supposed to have substantial amount of magnesium even after dilution but the absorbance of all samples turned out to be near the blank value. The standards were fine by the way. I’m wondering if this kit is not intended to be used for acidified samples?
This is an enzyme based assay which is dependent of the pH of the assay buffer that is provided in the kit. Hence, if the samples are quite acidic it was hamper the reaction mechanisms of the kit.
Can this kit be used with samples like bacteria, plants, drosophila, yeast etc?
We have optimized the kit with mammalian samples. However, theoretically these kits should work with samples from multiple species/sources. Since the optimal conditions depend on the sample type, the protocol has to be be adapted to fit the samples for efficient results. Please refer to this kit's citations to see what kind of samples have been used with this kit other than mammalian samples.
Can we use frozen samples with this assay?
Fresh samples are always preferred over frozen samples. However, frozen samples can also be used, provided, they were frozen right after isolation, were not freeze thawed multiple time (for which we recommend aliquoting the samples before freezing) and have been frozen for relatively short periods.
Can we use a different wavelength than recommended for the final analysis?
It is always recommended to use the exact recommended wavelength for the most efficient results. However, most plate readers have flexibility in their band width of detection in increments of +/- 10 nm. Depending on this flexibility range, you can deviate from the recommended wavelengths within limits.
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
What is the shelf life of this kit?
This kit is good for 12 months from the date of shipment in the unopened form when stored at the appropriate temperature and appropriate conditions. After opening and reconstitution, some of the components in this kit are good for 2 months at -20°C. Please refer to the datasheet for storage information and shelf life of each of the components.
Why are my standard curve values lower than those shown on the datasheet?
There are multiple factors which influence the signals like the incubation times, room temperature, handling etc. In general, to increase the value of the standards, you can increase the incubation time. As long as the standard curve is linear, it should be fine to use, since all of your samples will also be measured under the same conditions on this curve.
How do I normalize my samples against protein concentration
You can use a protein quantitation assay on the supernatants you get from cell/tissue lysates or with any other liquid sample in the assay buffer.
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.
Should I make a standard curve for every expt I do, or is one curve/kit enough?
Yes, I would strongly recommend you to do the standards every time you do the expt. There is always a chance that something was done differently that day and we do not want any conditions to differ between standards and samples.
Forke,K. J. et. al. DIABETES & GLUCOSE HOMEOSTASIS: GENETIC & TRANSLATIONAL APPROACHES - BASIC/TRANSLATIONAL: D-Lactate and Its Metabolic Intermediates as Potential Biomarkers for Diabetic Vascular Complications. Endocr. Rev., Jun 2011; 32: P2-536.
For more citations of this product click here
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