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Lysozyme Inhibitor Screening Kit

HTS, includes Lysozyme inhibitor
Catalog #: K237
$525.00

Product Details

Cat # +Size K237-100
Size 100 assays
Detection Method Fluorescence (Ex/Em = 360/445 nm)
Species Reactivity Mammalian
Applications The kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput screening of Lysozyme inhibitors. Inhibitor control (N,N',N''-Triacetylchitotriose) is included to compare the efficacy of test inhibitors.
Features & Benefits • Simple procedure
• Fast and convenient
• The assay is sensitive, stable and high-throughput adaptable
Kit Components • Lysozyme Assay Buffer
• Lysozyme substrate (in DMSO)
• Lysozyme (lyophilized)
• N,N',N''-Triacetylchitotriose
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Lysozyme (EC3.2.1.17), also known as muramidase or N-acetylmuramide glycanhydrolase, is a glycoside hydrolase. It is ubiquitously found in a wide range of biological fluids such as tears, saliva, serum, where it serves as a key effector of the innate immune system. It is also synthesized by certain carcinomas. The excessive production of lysozymes by cancer cells (especially myelomonocytic leukemia) results in higher levels of lysozyme causing renal failure. It has been demonstrated that the expression level of lysozyme is an independent prognostic factor which can be used to predict both relapse-free survival and overall survival in patients with breast carcinomas. Therefore, lysozyme inhibitors may constitute attractive, potential targets for developing anti-inflammatory and/or anti-tumor drugs. BioVision’s Lysozyme Inhibitor Screening Kit uses N,N′,N′′-Triacetylchitotriose -a competitive inhibitor that binds to the active site of lysozyme. The inhibitor decreases the catalytic ability of lysozyme to hydrolyze a fluorogenic substrate. The kit provides a simple, rapid, sensitive and reliable test suitable for screening of lysozyme inhibitors.


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Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.