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Lysosomal Cytotoxicity Dual Staining Kit (Cell-Based)

Catalog #: K458

In stock

$445.00

Product Details

Cat # +Size K458-50
Size 50 assays
Kit Summary • Detection method- Flow cytometry and Fluorescence Microscopy
• Species reactivity- Mammalian
• Applications - Measurement of Lysosomal cytotoxicity
- Screening compounds affecting lysosomal function
Detection Method Flow Cytometry (FL1/FL2)
Species Reactivity Mammalian
Applications • Measurement of lysosomal cytotoxicity.
• Staining for lysosome cytotoxicity and cell death.
• Screening for compounds that affect lysosomal function
Features & Benefits Measurement of lysosomal cytotoxicity.
Kit Components • Assay Buffer (10X)
• Lysosomal Stain (500X)
• Cell Death Marker (500X)
• Positive Control Reagent
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Lysosomes are membrane-bound organelles important for various cellular processes. They contain hydrolytic enzymes utilized in the metabolism of some biomolecules. The extracellular cargo (e.g. nutrients, toxins) binds to the cell membrane and is subsequently transported into membrane-bound endosomes for further degradation by lysosomes while intracellular components are transported to lysosomes through autophagy. Lysosomal dysfunction is associated with many human conditions such as aging and neurodegenerative disease. BioVision has developed the Lysosomal Cytotoxicity Dual Staining Kit (cell-based) which contains two probes; membrane permeable, selective Lysosomal Stain and Cell Death Marker. We also include a Positive Control Reagent, which increases lysosome activity and staining, thus serves as an experimental control. This easy-to-use, non-radioactive kit allows studying the regulation of lysosome and cytotoxicity at the cellular level by using Fluorescence Microscopy and Flow Cytometry in cultured cells.


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