Luciferase Reporter Assay Kit

Highly Sensitive Assay
Catalog #: K801 | abID: ab287865

Product Details

abID ab287865
Cat # +Size K801-200
Size 200 assays
Detection Method Luminescence
Species Reactivity Mammalian
Applications This Luciferase Reporter Assay Kit provides a simple means for detecting luciferase activity in transfected eukaryotic cells.
Features & Benefits • The assay ensures maximal sensitivity, consistent light output, as well as accuracy and consistency when working with multiple samples.
Kit Components • Substrate A
• Substrate B
• Cell Lysis Buffer
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Firefly luciferase has been used as a sensitive reporter for studying gene regulation and function. Luciferase provides a 1000-fold increase in sensitivity in comparison to the standard chloramphenicol acetyltransferase (CAT) assay. Firefly luciferase catalyzes the oxidative carboxylation of luciferin, a reaction with the highest efficiency of any known bioluminescence reaction. The light emission from the reaction can be recorded using a luminometer. BioVision’s Luciferase Reporter Assay Kit provides a simple means for detecting luciferase activity in transfected eukaryotic cells. For measurement of expressed luciferase in vitro, luciferase is first extracted from transfected cells through cell lysis. Then CoA, ATP, Mg²ᶧ and buffer are added to the lysate (Inclusion of CoA yields a nearly constant light emission rather than typical flash Kinetics). The luminescent reaction is then triggered by an injection of luciferin, and the emitted light is recorded by a luminometer. By providing CoA, along with ATP, Mg²ᶧ in an optimized buffer solution, BioVision’s Luciferase Reporter Assay Kit ensure maximal sensitivity, consistent light output, as well as convenience and consistency when working with multiple samples.


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