Product Details
| abID | ab65470 |
|---|---|
| Cat # +Size | K501-100 |
| Size | 100 stainings |
| Detection Method | Fluorescent microscopy (Ex/Em 488/518 nm) to detect staines live cells; (Ex/Em 488/615) to detect stained dead cells |
| Species Reactivity | Mammalian |
| Applications | Stained live and dead cells can easily be visualized by fluorescence microscopy. |
| Features & Benefits | • Simple procedure; takes less than 20 minutes • Fast and convenient |
| Kit Components | • Solution A (1 mM Live-Dye) • Solution B (1 mg/ml PI) • Staining Buffer |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
The assay range is 0.156–10 ng/ml ANGPTL3/ml. The lowest level of ANGPTL3 that can be detected by this assay is 75 pg/ml.
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Why do living cells fluorescence at the predetermined excitation range for the dead cells (propidium iodide)?
The living stain dye does have 10-20% emission tail in 615nm-therefore if excited at 488nm this dye will show a signal with PI filter set especially if the staining is very strong.
Can you please explain the principle of staining?
The green dye stain can permeate cell membrane of live eukaryotic cells and stains the cell green. PI can only label dead cells, since it is membrane impermeable.
Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
What is the shelf life of this kit?
BioVisions’s assay kits expire 6 months from the date of shipment. Some components of the kit may expire sooner as mentioned in the data sheet
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.
Yoshioka, Junya et al. (2016) Alternation of Gene Expression Levels in Mesenchymal Stem Cells by Applying Positive Dielectrophoresis, Anal Sci. 2016;32(11):1213-1216.
Sharma et al., Surface-Modified Electrospun Poly(
-Caprolactone) Scaffold With Improved Optical Transparency and Bioactivity for Damaged Ocular Surface Reconstruction. Invest. Ophthalmol. Vis. Sci., Feb 2014; 55: 899 - 907.
-Caprolactone) Scaffold With Improved Optical Transparency and Bioactivity for Damaged Ocular Surface Reconstruction. Invest. Ophthalmol. Vis. Sci., Feb 2014; 55: 899 - 907.Vogt KSC ET AL (2008) J. Exp. Biol. 211: 731-740.
For more citations of this product click here
