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Lipoxin B4 ELISA Kit

A Competitive ELISA kit for in vitro quantitative determination of Lipoxin B4 concentrations in serum, plasma and other biological fluids.
Catalog #: E4795
$545.00

Product Details

Size 96 assay
Detection Method Absorbance (450 nm)
Species Reactivity All
Applications in vitro quantitative determination of LXB4 concentrations in serum, plasma and other biological fluids.
Features & Benefits • Sensitivity: 0.19 ng/mL
• Precision: Coefficient of variation is < 10%.
• Specificity: No Significant cross-reactivity or interference between LXB4 and analogues was observed.
• Detection Range: 0.31-20 ng/mL
Kit Components • Micro ELISA Plate
• Reference Standard
• Biotinylated Detection Ab (100x)
• HRP Conjugate (100x)
• Reference Standard & Sample Diluent
• Biotinylated Detection Antibody Diluent
• HRP Conjugate Diluent
• Wash Buffer (25X)
• Substrate Reagent
• Stop Solution
• Plate Sealer
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Lipoxin A4 (LXA4) and Lipoxin B4 (LXB4) biosynthesis has been detected in a variety of inflammatory conditions. Lipoxin B4 (LXB4) is a member of the lipoxin family, with its proresolving effects shown in allergic airway inflammation. BioVision’s Lipoxin B4 ELISA Kit is based on Competitive ELISA principle. The micro-plate provided in this kit has been pre-coated with Lipoxin B4. During the reaction, Lipoxin A4 in the samples or standard competes with Lipoxin B4 coated on the plate for binding to the anti-Lipoxin B4 antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of Lipoxin B4. The concentration of Lipoxin B4 in the samples can be calculated by comparing the OD of the samples to the standard curve.


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