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Lipoteichoic acid (LTA) (Human) ELISA Kit

A Sandwich ELISA kit to quantitatively measure Lipoteichoic acid (LTA) in human serum, plasma, tissue lysates, and other biological fluids

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which is [are] known to the State of California to cause birth defects or other reproductive harm.  For more information go to  www.P65Warnings.ca.gov.
Catalog #: E4931
$695.00

Product Details

Cat # +Size E4931-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications This Sandwich ELISA kit is designed to quantitatively measure amount of LTA in human serum, plasma, tissue lysates, and other biological fluids
Features & Benefits • Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%
• Recovery range: 85 - 105% for normal human serum and plasma samples
• Sensitivity: 4.688 pg/ml
• This Sandwich ELISA is highly sensitive and highly specific for the detection of LTA in human samples. There is no significant cross-reactivity or interference between LTA and analogues
• Detection range: 7.813 - 500 pg/ml
Kit Components • Micro ELISA plate
• Standard (Lyophilized) (500 pg)
• Sample/Standard Dilution Buffer
• Biotin-labeled Antibody
• Antibody Dilution Buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC Dilution Buffer
• TMB Substrate Solution
• Stop Solution
• Wash Buffer (25X)
• Plate Sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Lipoteichoic acid (LTA) is an alditol phosphate-containing polymer that is anchored to the membrane via glycolipid. LTA is found only on the cell wall of Gram-positive bacteria. Upon stimulation by cationic peptides and lysozymes of leukocytes or β-lactam antibiotics, it is released from bacterial cells via bacteriolysis. LTA binds to target cells via either membrane phospholipids or CD14 and TLR2, thus resulting in the release of reactive oxygen species, bactericidal peptides, and proinflammatory cytokines and amplifying cell damage. Target cell-bound LTA can also bind to circulating antibodies and activate the complement cascade, resulting in a passive immune kill phenomenon. LTA is implicated in the pathophysiology of inflammation and postinfectious sequelae such as septic shock, adult respiratory distress syndrome (ARDS), thus indicating that it is the major virulence factor in Gram-positive bacterial infections. BioVision’s Lipoteichoic acid (LTA) (Human) ELISA kit is based on the Sandwich ELISA principle to quantitatively measure the concentration of LTA in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is directly proportional to the concentration of LTA in the sample or standard.


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