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Lipopolysaccharide (LPS) Isolation Kit

Simple, one step assay to isolate PBMC
Catalog #: K438

In stock

$195.00

Product Details

Cat # +Size K438-10
Size 30 ml isolation
Kit Summary Isolation of LPS from outer membrane of gram negative bacteria.
Detection Method Not Applicable
Species Reactivity Bacteria
Applications Isolation of LPS from outer membrane of gram negative bacteria.
Features & Benefits •This kit does not use chlorofrom or phenol like traditional methods
• Yields pure LPS in less than 2 hours that can be easily characterized and quantified
Kit Components • LPS Isolation Buffer
• Proteinase K (20 mg/ml)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

The outer membrane of gram-negative bacteria contains lipopolysaccharide (LPS), a low molecular weight carbohydrate with a molecular mass of 10-20 kDa. It is heterogeneous, and composed of O antigen (a repeating glycan polymer), core oligosaccharide (which links the O antigen to Lipid A - the third component, and non-carbohydrate components such as phosphate and amino acids groups. Lipid A, has multiple fatty acids which serve to anchor LPS into the bacterial membrane allowing the O antigen and core oligosaccharide to protrude and contributes to a major part of the toxicity of gram-negative bacteria. Also known as endotoxin, when consumed by animals, LPS induces a strong inflammatory response and/or sepsis. BioVision’s LPS Isolation Kit uses bacterial membrane lysis buffer and protein digestion to yield micrograms of LPS from bacterial culture (approximately 1-4% of dry weight). This kit does not use chlorofrom or phenol like traditional methods, and it will yield pure LPS in less than 2 hours that can be easily characterized and quantified!


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