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Lipolysis (3T3-L1) Colorimetric Assay Kit

Highly Sensitive Assay
Catalog #: K577

In stock

$425.00

Product Details

Cat # +Size K577-100
Size 100 assays
Detection Method Absorbance (OD 570 nm)
Applications Measurement of lipolysis in 3T3-L1 cells or adipocytes
Screening compounds that influence lipolysis, mechanistic studies, and studies on metabolic dysfunctions
Features & Benefits • Simple, rapid & convenient
• The assay can detect less than 200 pmol of glycerol.
Kit Components • Lipolysis Assay Buffer
• Lipolysis Wash Buffer
• Glycerol Assay Buffer
• Glycerol Probe (in DMSO, Anhydrous)
• Glycerol Enzyme Mix (Lyophilized)
• Glycerol Standard (100 mM)
• Isoproterenol (10 mM)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Lipolysis is the hydrolysis of triglycerides within the cell into glycerol and free fatty acids. The glycerol and free fatty acids are then released into the bloodstream or culture media. Lipolysis occurs in essentially all cells, but is most abundant in white and brown adipose tissue. Deficiencies in lipolysis lead to increased intracellular lipid accumulation, resulting in abnormal cellular physiology, hyperlipidemia, and insulin resistance. Lipolysis can be induced by catecholamines and certain hormones. The kit includes synthetic catecholamines, Isoproterenol, which activates β-adrenergic receptors. This leads to activation of adenylate cyclase, which catalyzes the conversion of ATP to cAMP. cAMP then serves as a second messenger to activate hormone-sensitive lipase, which hydrolyzes the triglycerides. This pathway can be inhibited by insulin. BioVision’s 3T3-L1 Lipolysis Assay kit is simple and easy-to-use. The assay measures glycerol released from 3T3-L1 cells after induction of lipolysis using colorimetric method. The color intensity is directly proportional to the amount of glycerol. This assay kit can detect less than 200 pmol of glycerol.


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Can phenol red interfere with the assay?
Red color from media can affect the OD measurement at 570 nm, depending on the volume added per well. The volume of the media sample with phenol red should be limited to 20ul
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
What is the shelf life of this kit?
 BioVisions’s assay kits expire 6 months from the date of shipment. Some components of the kit may expire sooner as mentioned in the data sheet
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.
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