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Lipid (Oil Red O) staining Kit

Catalog #: K580

In stock

$345.00

Product Details

Cat # +Size K580-24
Size 24 ml
Detection Method Microscopy
Applications Staining of neutral lipids in cells.
Features & Benefits • Simple, rapid & convenient method to selectively detect neutral lipids within cultured cells.
• Includes Hematoxylin to stain nuclei
Kit Components • PBS
• Formalin (10%)
• Oil Red O
• Hematoxylin
Storage Conditions RT
Shipping Conditions RT
USAGE For Research Use Only! Not For Use in Humans.

Details

Lipid droplets are found in all eukaryotic organisms. They function as reservoirs of neutral lipids, primarily triacylglycerol and cholesterol esters. The stored lipids can be used for energy, steroid synthesis, or membrane formation. Lipid droplet accumulation is a normal function in cells. Adipocytes consist predominantly of lipid droplets; however excessive lipid droplet accumulation within cells can be an indicator of metabolic deficiency or pathogenesis. For example, excessive accumulation of lipids in liver cells (steatosis) can lead to cellular dysfunction. At the onset of atherosclerosis, macrophages engulf oxidized-LDL, develop into foam cells, and contribute to artery narrowing. BioVision’s Lipid (Oil Red O) Staining Kit allows selective detection of neutral lipids within cultured cells. The kit also includes Hematoxylin to stain nuclei. This kit provides enough reagents to stain two (2) 96-well plates, two (2) 6-well plates, or four (4) 100 mm culture dishes.


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Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
What is the shelf life of this kit?
 BioVisions’s assay kits expire 6 months from the date of shipment. Some components of the kit may expire sooner as mentioned in the data sheet
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.
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