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Lipase Activity Fluorometric Assay Kit III

Highly Sensitive Assay, HTS
Catalog #: K724

In stock

$410.00

Product Details

Cat # +Size K724-100
Size 100 assays
Detection Method Fluorescence (Ex/Em 529/600 nm)
Species Reactivity Mammalian
Applications A sensitive and high speed homogeneous assay for detection of Lipase enzyme activity in a high-throughput screening (HTS) format.
Features & Benefits • Simple procedure; takes ~ 60 minutes
• Fast and convenient
• Sensitive assays for measuring Lipase activity as low as 0.1 mU/well.
Kit Components • Lipase Assay Buffer
• Lipase Substrate
• Methylresorufin Standard (0.1 mM)
• Lipase Positive Control
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Lipase is a subclass of the esterases that catalyze the hydrolysis of ester bonds of lipid substrates. Lipases perform essential roles in the digestion, transport and processing of dietary lipids (e.g. triglycerides, fats, oils) in most, if not all, living organisms. In humans, pancreatic lipase is the key enzyme responsible for breaking down fats in the digestive system by converting triglycerides to monoglycerides and free fatty acids. During the damage of the pancreas, lipase levels can rise 5 to 10-fold within 24 to 48 hours. In BioVision’s Lipase Assay Kit III, Lipase hydrolyzes a specific substrate to generate the methylresorufin, which can be detected fluorometrically at Em/Ex=529/600 nm. The kit provides a rapid, simple, more sensitive, and reliable test suitable for high throughput assay of Lipase activity. This kit can be used to detect Lipase as low as 0.1 microU/well.


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What is the source of the positive control?
The positive control is isolated from Chromobacteritim viscosum.
What can interfere with the assay?
Mercaptans, DTT, EDTA, NaCl, PMSF, SDS, Triton-X in samples can interfere with the assay.
How do we collect blood for this assay?
Blood should be collected in heparin tubes and EDTA/metal chelators should be avoided.
Can we use frozen samples with this assay?
We would recommend using fresh samples. However, frozen samples can be used too provided they have not undergone multiple freeze thaw cycles.
Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only.
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
What is the shelf life of this kit?
BioVisions’s assay kits expire 6-12 months from the date of shipment. Some components of the kit may expire sooner as mentioned in the data sheet.
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.