Lipase Assay Kit (Colorimetric) (ab102524)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr 30 min
- Sample type: Cell culture media, Cell culture supernatant, Milk, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.02 mU/well
Overview
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Product name
Lipase Assay Kit (Colorimetric)
See all Lipase kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Milk, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell culture media -
Assay type
Enzyme activity -
Sensitivity
0.02 mU/well -
Assay time
1h 30m -
Product overview
Lipase Assay Kit ab102524 is a rapid, simple, and sensitive colorimetric assay for the measurement of lipase activity.
In the lipase assay protocol, lipase hydrolyzes a triglyceride substrate to form glycerol which is quantified enzymatically by monitoring a linked change in the absorbance of a probe (OD=570nm). This kit is suitable for high throughput analysis (HTP).
This lipase assay kit detects lipase activity as low as 0.02mU per well.
Lipase assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze every 2-3 min with microplate reader in kinetic mode for at least 60-90 min at 37ºC -
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K722 Lipase Activity Colorimetric Assay Kit. K722-100 is the same size as the 100 test size of ab102524.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Assay Buffer V 1 x 25ml Enzyme Mix VI 1 vial Glycerol Standard 1 x 0.2ml Lipase Positive Control 1 vial Lipase Substrate III 1 vial OxiRed Probe 1 x 0.2ml -
Research areas
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Relevance
Lipases perform essential roles in the digestion, transport and processing of dietary lipids (e.g. fats and oils) in living organisms. In humans, pancreatic lipase is the key enzyme responsible for breaking down fats in the digestive system by converting triglyceride to monoglyceride and free fatty acid. Pancreatic lipase monitoring is also used to help diagnose Crohn's disease, cystic fibrosis and celiac disease. Damage to the pancreas can exhibit a 5-10 fold increase of serum lipase levels within 24 to 48 hours.
Associated products
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Related Products
Images
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Plasma lipase levels were measured (using ab102524) after 75 days treatment with saline, liraglutide, exendin-4 or sitagliptin. ND, normal chow diet; HFD, high fat diet. p≤0.05, *; p≤0.01, **, n = 3–7 mice.
There were no detectable differences in plasma lipase activity in mice on a normal chow diet administered any of the three drugs when compared to animals administered saline. Likewise, there was no significant change in plasma lipase activity in mice that were administered saline on a high fat diet vsnormal diet. Furthermore, administration of liraglutide and exendin-4 in combination with a high fat diet also failed to affect plasma lipase activity. We observed no detectable changes in plasma lipase activity in animals maintained on a normal chow diet and administered any of the three drugs when compared to animals administered saline.
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Sample Timeline
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Glycerol standard Curve
Datasheets and documents
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SDS download
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Datasheet download
References (21)
ab102524 has been referenced in 21 publications.
- Bartolacci C et al. Targeting de novo lipogenesis and the Lands cycle induces ferroptosis in KRAS-mutant lung cancer. Nat Commun 13:4327 (2022). PubMed: 35882862
- Tung YT et al. Ascophyllum nodosum and Fucus vesiculosus Extracts Improved Lipid Metabolism and Inflammation in High-Energy Diet-Induced Hyperlipidemia Rats. Nutrients 14:N/A (2022). PubMed: 36364926
- Arévalo Sureda E et al. Exocrine Pancreatic Maturation in Pre-term and Term Piglets Supplemented With Bovine Colostrum. Front Nutr 8:687056 (2021). PubMed: 34249996
- Dolai S et al. Pancreas-specific SNAP23 depletion prevents pancreatitis by attenuating pathological basolateral exocytosis and formation of trypsin-activating autolysosomes. Autophagy 17:3068-3081 (2021). PubMed: 33213278
- Wu J et al. Treatment of Severe Acute Pancreatitis and Related Lung Injury by Targeting Gasdermin D-Mediated Pyroptosis. Front Cell Dev Biol 9:780142 (2021). PubMed: 34858995