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Leukotriene B4 ELISA Kit

A Competitive ELISA kit for in vitro quantitative determination of Leukotriene B4 concentrations in serum, plasma and other biological fluids.

WARNING: This product can expose you to chemicals including TMB, which is [are] known to the State of California to cause cancer.  For more information go to
Catalog #: E4793

Product Details

Size 96 assay
Detection Method Absorbance (450 nm)
Species Reactivity All
Applications in vitro quantitative determination of Leukotriene B4 concentrations in serum, plasma and other biological fluids.
Features & Benefits • Detection Range: 15.63-1000 pg/mL
• Specificity: This kit recognizes LTB4 in samples. No Significant cross-reactivity or interference between LTB4 and analogues was observed.
• Sensitivity: 9.38 pg/mL
• Precision:Coefficient of variation is < 10%.
Kit Components • Micro ELISA Plate
• Reference Standard
• Biotinylated Detection Ab (100x)
• HRP Conjugate (100x)
• Reference Standard & Sample Diluent
• Biotinylated Detection Antibody Diluent
• HRP Conjugate Diluent
• Wash Buffer (25X)
• Substrate Reagent
• Stop Solution
• Plate Sealer
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Leukotriene B4 (LTB4) is a pro-inflammatory mediator synthesized in myeloid cells from arachidonic acid. Leukotrienes are lipid mediators whose production is increased during inflammation. Leukotriene B4 stimulates leukocyte functions including lysosomal enzyme release, adhesion, and aggregation of polymorphonuclear leukocytes. LTB4 has been implicated as a potent mediator of inflammatory diseases and immunoregulation. BioVision’s LTB4 ELISA Kit is based on Competitive ELISA principle. The micro-plate provided in this kit has been pre-coated with LTB4. During the reaction, LTB4 in the samples or standard competes with LTB4 coated on the plate for binding to the anti-LTB4 antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of LTB4. The concentration of LTB4 in the samples can be calculated by comparing the OD of the samples to the standard curve.

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