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New

LDL Uptake Assay Kit (Cell-Based)

Sensitive assay for the measurement of LDL
Catalog #: K436

In stock

$555.00

Product Details

Cat # +Size K436-100
Size 100 assays
Kit Summary Easy-to-follow protocol for the measurement of LDL uptake in mammalian cell lines.
Detection Method Fluorometric (Ex/Em= 488/523 nm)
Species Reactivity All
Applications Screening and characterization of drugs or treatment conditions that inhibit or stimulate LDL uptake,Characterization of LDL uptake in different cell types.
Features & Benefits • Measure LDL uptake in different cell types
• Non-Radioactive
• Contains fluorescent human LDL
• which is covalently-labeled with a bright
• highly photostable fluorophore
• Screen
• study or characterize stimulators or inhibitors of LDL uptake
Kit Components • Flurophore-Labeled LDL (5 mg/ml)
• Unlabeled LDL
• Polylysine-Coated Cell Culture Plate
• Uptake Assay Buffer
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Low-density lipoprotein (LDL) is a large spherical macromolecule that serves as a ‘shuttle’ to transport hydrophobic molecules (lipids and cholesterol) throughout the body via the aqueous environment of the bloodstream. An LDL particle consists of a hydrophobic core of cholesterol esters (with long-chain fatty acids linked to the hydroxyl group) and triglycerides surrounded by an amphipathic shell containing the 510 kDa protein Apolipoprotein B-100, various phospholipids and non-esterified cholesterol molecules. LDL is taken up by cells such as hepatocytes, macrophages, and endothelial cells by specific receptor-mediated endocytosis. High levels of LDL have been associated with arterial inflammation, which leads to cardiovascular diseases (CVD) like atherosclerosis, ischemic stroke and myocardial infarction. LDL particles can migrate through inflamed vascular endothelial tight junctions, where they become oxidized and attract circulating monocytes. Monocytes differentiate into activated macrophages, which further accumulate LDL and become foam cells, leading to arterial wall thickening/scaring and formation of fibrous fatty plaques. Current CVD preventative therapies include decreasing LDL with statins, which prevent endogenous cholesterol biosynthesis by inhibiting HMG-CoA reductase. However, drugs that inhibit LDL uptake by macrophages or enhance LDL clearance by increasing hepatic LDL reuptake are also being investigated. BioVision’s LDL Uptake Assay Kit enables measurement of receptor-mediated LDL uptake by various cell types. The kit contains fluorescent human LDL, which is covalently-labeled with a bright, highly photostable fluorophore that can be detected when taken up by cells (Ex/Em = 488/523 nm). Unlabeled human LDL is included in the kit for validation of specific labeled LDL uptake. The kit contains a complete set of reagents sufficient for performing 100 reactions and a 96-well polylysine-coated black cell culture plate with a thin, microscopy-grade clear bottom.


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