Product Details
Cat # +Size | K311-400 |
---|---|
Size | 400 assays |
Detection Method | Absorbance (500 nm) |
Species Reactivity | Mammalian |
Applications | This assay provides a convenient and non-radioactive alternative to the [3H]-thymidine and the [51Cr] release assays The assay is based on measurement of activity of lactate dehydrogenase (LDH) which is a stable enzyme normally found in the cytosol of all cells but rapidly releases into the supernatant upon damage of plasma membrane. |
Features & Benefits | • Simple one-step procedure; takes less than 1 hour • Fast, sensitive and convenient |
Kit Components | • Catalyst, Lyophilized • Dye Solution |
Storage Conditions | -20°C |
Shipping Conditions | Gel Pack |
USAGE | For Research Use Only! Not For Use in Humans. |
Details
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1) Maybe the cells do not contain LDH;
2) Maybe the Triton X-100 concentration was not correct, or the low control cells were also died. Generally, low control should have an OD reading 0.5-1, whereas high control has an OD reading 2-3.
3) The machine filter setting might not be correct.