Product Details
abID | ab65393 |
---|---|
Cat # +Size | K313-500 |
Size | 500 assays |
Detection Method | Absorbance (450 nm) |
Species Reactivity | Mammalian |
Applications | This assay provides a convenient and Wst-based non-radioactive assay for measurement of activity of lactate dehydrogenase (LDH) which is a stable enzyme normally found in the cytosol of all cells but rapidly releases into the supernatant upon damage of plasma membrane. |
Features & Benefits | • Simple one-step procedure; takes around than 1 hour • Fast and convenient |
Kit Components | • WST Substrate Mix • LDH Assay Buffer • Cell Lysis Solution • Stop Solution • LDH (0.1 µg/ml) |
Storage Conditions | -20°C |
Shipping Conditions | Gel Pack |
USAGE | For Research Use Only! Not For Use in Humans. |
Details
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2. If, yes, how?
3. the plating density of the cells is fixed at 3000 cells/mm^2. Does she have to plate the cells using a 96-well plate? May she use 24-well, 12-well plate, and a microelectrode array chamber, then at day 7,14,21, and 28, she could take out some supernatant to a 96-well plate to assay?
4. if this is ok, may I store the supernatants taken out on different days, store them at -20C or -80C, then perform the LDH assay at one time?