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LATS1 (Human) ELISA Kit

A Sandwich ELISA kit for quantitative measurement of LATS1 in human serum, plasma, tissue lysates, and other biological fluids

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E5024
$795.00

Product Details

Cat # +Size E5024-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications Sandwich ELISA kit for quantitative measurement of DECTIN-1 in serum, plasma, cell and tissue lysates, and other biological fluids
Features & Benefits ● Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%
● Sensitivity: 0.094 ng/ml
● This Sandwich ELISA is highly sensitive and highly specific for the detection of LATS1 in human samples. There is no significant cross-reactivity or interference between LATS1 and analogues
● Recovery range: 85 - 105% for normal human serum and plasma samples
● Detection range: 0.156 – 10 ng/ml
Kit Components ● Micro ELISA plate
● Wash Buffer (25X)
● Plate sealers
● Standard (Lyophilized) (5000 pg)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate Solution
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Large tumor suppressor 1 (LATS1) is a serine/threonine kinase that belongs to the AGC kinase family of proteins. It is a core component of the Hippo signaling pathway in mammals. MST1/2 together with SAV1 phosphorylates and activates LATS1/2 and MOB1, which in turn phosphorylate YAP1 and TAZ. Phosphorylation of YAP1 by LATS1/2 prevents its translocation to the nucleus and thereby inhibits cell proliferation, migration, and cell death. LATS1 also regulates other biological processes such as cell cycle progression, apoptosis, renewal, and differentiation of stem cells. It acts as a tumor suppressor; studies demonstrated that loss of LATS1, deficiency of LATS1, or mutation in LATS1 reduced YAP1 phosphorylation and promotes tumor growth in multiple cancers. Hence LATS1 could be a promising target for anti-cancer therapies. BioVision’s LATS1 (Human) ELISA kit quantitatively measures LATS1 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. HRP-Streptavidin is added to the plate, and post-incubation any unattached conjugates are washed off by Wash Buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of LATS1 in the sample or standard.


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