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L-Arginine Assay Kit (Colorimetric)

First assay in the market that measures Arginine in biological Samples
Catalog #: K749
$515.00

Product Details

Cat # +Size K749-100
Size 100 assays
Kit Summary • Detection method- Absorbance (450 nm)
• Species reactivity- Eurkayotes and Prokaryotes
Detection Method Absorbance (OD 450 nm)
Species Reactivity Eukaryotes and Prokaryotes
Applications Measurement of Arginine in biological samples
Features & Benefits • Simple procedure; takes ~ 120 minutes
• Fast and convenient
• Kit contains the necessary reagents for accurate measurement of Arginine
Kit Components • Arginine Assay Buffer
• Arginine Enzyme Mix
• Arginine Probe Mix A
• Arginine Probe Mix B
• Sample Cleanup Mix
• Arginine Standard
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

L-Arginine (Arg) is a proteogenic, semi-essential amino acid: healthy humans can synthesize L-Arginine using L-Glutamine as a building block; however, premature infants are unable to produce Arg and additional supplementation is required for proper growth and development. Arginine plays pivotal roles in biochemical pathways such as the urea cycle and the biosynthesis of nitric oxide. Arginine and Ammonia concentrations are elevated in patients having a mutation in their ARG1 genes. The mutation causes lower arginase activities – a condition that is known as Argininemia. Arginine has also been advertised as a supplement due to its role in the synthesis of nitric oxide., which helps in vasodilation processes. BioVision’s L-Arginine Assay Kit provides a quick, specific, and easy method for the measurement of total L-arginine concentrations in a wide variety of samples. In this enzyme-based assay, L-arginine is converted into a series of intermediates, which will further react with a probe producing a stable colorimetric signal (OD: 450 nm). The kit is simple to use, sensitive and high-throughput adaptable and can detect as low as 1 nmol/well of L-arginine in biological samples


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What is the source of the Arginine enzyme used in the assay ?
It is purified from bovine liver.
Can the K749-100 L-Arginine Assay Kit be used with tissue or cell extracts? Do you have a recommended extraction buffer? Should the samples be put through the 10 kDa spin column before the assay?
We have not tested the kit with cell or tissue extracts. Theoretically the kit should work with those sample types. For sample preparation, please homogenize 1x10^6 cells or 10-50 mg tissue in 100 µl of cold assay buffer. Homogenize using either a dounce homogenizer or sonicate for few short pulses on ice. Centrifuge at 10,000g for 10 minutes. Take the supernatant and filter through a 10 kDa spin filter to deproteinize. You can also use more cells/tissue if needed, but please remember to increase the volume of assay buffer proportionally for homogenization.
Xazmin H. Lowman, p53 Promotes Cancer Cell Adaptation to Glutamine Deprivation by Upregulating Slc7a3 to Increase Arginine Uptake. Cell Rep, March 2019;  30865893.
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