Call 408.493.1800 | Fax 408.493.1801 | Toll Free 800.891.9699 (US Only) | Email: [email protected]

New

KL-6 (Human) ELISA Kit

A Sandwich ELISA kit to quantitatively measure KL-6 in human serum, plasma, tissue lysates, and other biological fluids
Catalog #: E4892
$695.00

Product Details

Cat # +Size E4892-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications This Sandwich ELISA kit is designed to quantitatively measure amount of KL-6 in human serum, plasma, tissue lysates, and other biological fluids
Features & Benefits • Recovery range: 85 - 105% for normal human serum and plasma samples
• Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%
• This Sandwich ELISA is highly sensitive and highly specific for the detection of KL-6 in human samples. There is no significant cross-reactivity or interference between KL-6 and analogues
• Detection range: 0.156 - 10 ng/ml
• Sensitivity: 0.094 ng/ml
Kit Components • Micro ELISA plate
• Standard (Lyophilized) (10 ng)
• Sample/Standard Dilution Buffer
• Biotin-labeled Antibody
• Antibody Dilution Buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC Dilution Buffer
• TMB Substrate Solution
• Stop Solution
• Wash Buffer (25X)
• Plate Sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Krebs von den Lungen-6 (KL-6) is a high molecular weight glycoprotein that belongs to the MUC-1 family. Moderate KL-6 expression is observed in type II pneumocytes and respiratory bronchiolar epithelial cells, whereas weak KL-6 expression is observed in basal cells of the terminal bronchiolar epithelium in normal lung tissues. Several studies reported increased KL-6 levels in regenerated type II alveolar epithelial cells and the serum of patients suffering from interstitial lung disease (ILD). Hence, KL-6 could serve as an efficient therapeutic target for the treatment of pulmonary diseases such as idiopathic pulmonary fibrosis, CTD-associated ILD, cystic fibrosis, and hypersensitivity pneumonitis. BioVision’s KL-6 (Human) ELISA kit is based on the Sandwich ELISA principle to quantitatively measure the amount of KL-6 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of KL-6 in the sample or standard.


Why buy BioVision Products?
Innovation
Affordability
Global Presence
Technical Support
BioVision aims to provide our customers innovative tools for accelerating drug discovery and biological research. BioVision offers >8,000 products including the most comprehensive array of assay kits for key targets in Metabolic pathways.
BioVision is committed to providing the highest quality products at a competitive price.
We have a broad network of global distributors who are ready to address your research needs and ensure fast delivery.
Our highly trained Technical Support team provides comprehensive product support and is dedicated to resolving your issues quickly and efficiently.