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KL-6 (Human) ELISA Kit

A Sandwich ELISA kit to quantitatively measure KL-6 in human serum, plasma, tissue lysates, and other biological fluids
Catalog #: E4892

Product Details

Cat # +Size E4892-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications This Sandwich ELISA kit is designed to quantitatively measure amount of KL-6 in human serum, plasma, tissue lysates, and other biological fluids
Features & Benefits • Recovery range: 85 - 105% for normal human serum and plasma samples
• Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%
• This Sandwich ELISA is highly sensitive and highly specific for the detection of KL-6 in human samples. There is no significant cross-reactivity or interference between KL-6 and analogues
• Detection range: 0.156 - 10 ng/ml
• Sensitivity: 0.094 ng/ml
Kit Components • Micro ELISA plate
• Standard (Lyophilized) (10 ng)
• Sample/Standard Dilution Buffer
• Biotin-labeled Antibody
• Antibody Dilution Buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC Dilution Buffer
• TMB Substrate Solution
• Stop Solution
• Wash Buffer (25X)
• Plate Sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Krebs von den Lungen-6 (KL-6) is a high molecular weight glycoprotein that belongs to the MUC-1 family. Moderate KL-6 expression is observed in type II pneumocytes and respiratory bronchiolar epithelial cells, whereas weak KL-6 expression is observed in basal cells of the terminal bronchiolar epithelium in normal lung tissues. Several studies reported increased KL-6 levels in regenerated type II alveolar epithelial cells and the serum of patients suffering from interstitial lung disease (ILD). Hence, KL-6 could serve as an efficient therapeutic target for the treatment of pulmonary diseases such as idiopathic pulmonary fibrosis, CTD-associated ILD, cystic fibrosis, and hypersensitivity pneumonitis. BioVision’s KL-6 (Human) ELISA kit is based on the Sandwich ELISA principle to quantitatively measure the amount of KL-6 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of KL-6 in the sample or standard.

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