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KEAP1 (Human) ELISA Kit

A Sandwich ELISA kit for quantitative measurement of KEAP1 in human serum, plasma, tissue lysates, and other biological fluids

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E5021
$750.00

Product Details

Cat # +Size E5021-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications Sandwich ELISA kit for quantitative measurement of KEAP1 in serum, plasma, cell and tissue lysates, and other biological fluids
Features & Benefits ● Detection range: 23.438 – 1500 pg/ml
● Recovery range: 85 - 105% for normal human serum and plasma samples
● Sensitivity: 14.063 pg/ml
● Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%
● This Sandwich ELISA is highly sensitive and highly specific for the detection of KEAP1 in human samples. There is no significant cross-reactivity or interference between KEAP1 and analogues
Kit Components ● Micro ELISA plate
● Wash Buffer (25X)
● Plate sealers
● Standard (Lyophilized) (5000 pg)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate Solution
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Kelch-like ECH-associated protein 1 (KEAP1) is a redox-sensitive protein that belongs to the family of BTB proteins. KEAP1 contains cysteine residues whose thiol groups play a significant role in binding KEAP1 with CUL3 protein and forms a Cullin-RING E3 ligase complex required for Nrf2 degradation. Under oxidative stress conditions, electrophiles and reactive oxygen species (ROS) modulate the thiol groups on KEAP1, thus preventing the binding with CUL3. As a result, NRF2 translocates to the nucleus and binds to antioxidant responsive elements (AREs), and activates the expression of antioxidant proteins. Activation of NRF2 has been demonstrated to be beneficial in diabetes, Parkinson’s, and Alzheimer’s’ disease. Mutations in KEAP1 are associated with solid tumors. BioVision’s KEAP1 (Human) ELISA kit quantitatively measures KEAP1 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. HRP-Streptavidin is added to the plate, and post-incubation any unattached conjugates are washed off by Wash Buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of KEAP1 in the sample or standard.


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