Call 408.493.1800 | Fax 408.493.1801 | Toll Free 800.891.9699 (US Only) | Email: [email protected]

Jurkat cell Lysate (Camptothecin-treated)

Camptothecin induced Jurkat cell extracts useful as a positive control in western blotting or caspase activity assays.
Catalog #: 2402
$95.00

Product Details

Cat # +Size 2402-100
Size 100 μg
Highlights • Concentration- 2 mg/ml in 1X SDS sample buffer containing 5% β-mercaptoethanol. • Application- The Jurkat cell lysate can be boiled for 5 minutes and then loaded onto SDS-PAGE for Western blotting. 20 µg/lane is recommended for mini gel.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Jurkat cells were treated with 2 mM camptothecin for 6 hours. Cell lysate was prepared by homogenization in modified RIPA buffer (150 mM NaCl, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. The cell lysate was then diluted to 1X SDS sample buffer (final concentration- 50 mM Tris-HCl, pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.


Why buy BioVision Products?
Innovation
Affordability
Global Presence
Technical Support
BioVision aims to provide our customers innovative tools for accelerating drug discovery and biological research. BioVision offers >8,000 products including the most comprehensive array of assay kits for key targets in Metabolic pathways.
BioVision is committed to providing the highest quality products at a competitive price.
We have a broad network of global distributors who are ready to address your research needs and ensure fast delivery.
Our highly trained Technical Support team provides comprehensive product support and is dedicated to resolving your issues quickly and efficiently.