JNK Activity Screening Kit, KinaseSTAR

Catalog #: K430 | abID: ab284544

Product Details

abID ab284544
Cat # +Size K430-40
Size 40 assays
Detection Method Western blot analysis with anti-Phospho-cJun (Ser 73) Specific Antibody at 1:1000 dilutions
Species Reactivity Mammalian
Applications The kit can be used to screen JNK activity relatively quickly for large numbers of samples.
Features & Benefits • Simple procedure
• The kit is suitable for both research and drug discovery
• The assay utilizes a N-terminal c-Jun fusion protein bead to selectively “pull down” JNK from cell lysate c-Jun phosphorylation is then measured using a phospho-c-Jun specific antibody.
Kit Components • Kinase Extraction Buffer
• c-Jun (1-79) Fusion Protein Beads
• Kinase Assay Buffer
• ATP (10 mM)
• Phospho-cJun Specific Antibody
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


c-Jun N-terminal kinase (JNK) is one of the several main MAP kinase groups identified in mammals. Recent evidences suggest that activation of JNK may play an important role in neuronal apoptosis and other physiological and pathological processes. The JNK Activity screening kit utilizes an N-terminal c-Jun (1-79) fusion protein bound to glutathion sepharose beads to selectively “pull down” JNK from cell lysates. After wash to remove nonspecifically bound proteins, the kinase reaction is then carried out in the presence of cold ATP. c-Jun phosphorylation is then measured by Western blot analysis using a phospho-c-Jun specific antibody.

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Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
What is the shelf life of this kit?
 BioVisions’s assay kits expire 6 months from the date of shipment. Some components of the kit may expire sooner as mentioned in the data sheet
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.
Yu, W. et al. Polycystin-1 Protein Level Determines Activity of the G 12/JNK Apoptosis Pathway. J. Biol. Chem. 2010 285: 10243-10251.
Yu XX et al (2008) Am J Physiol Endocrinol Metab 295: E436 - E446.
Shintani, Y. et al. (2006) Cancer Res. 66: 11745-11753.
For more citations of this product click here